BRENDA - Enzyme Database show
show all sequences of 1.13.11.B6

Dual positional specificity and expression of non-traditional lipoxygenase induced by wounding and methyl jasmonate in maize seedlings

Kim, E.S.; Choi, E.; Kim, Y.; Cho, K.; Lee, A.; Shim, J.; Rakwal, R.; Agrawal, G.K.; Han, O.; Plant Mol. Biol. 52, 1203-1213 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Zea mays
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
97000
-
1 * 97000, recombinant enzyme including His-tag, SDS-PAGE
Zea mays
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
Q9LKL4
cv. Golden Cross Bantam 70
-
Purification (Commentary)
Commentary
Organism
-
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Zea mays
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linolenate + O2
-
706251
Zea mays
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
the enzyme forms (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate and (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate in a 6:4 ratio. The R/S stereoconfiguration of the products is not determined
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 97000, recombinant enzyme including His-tag, SDS-PAGE
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Zea mays
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
97000
-
1 * 97000, recombinant enzyme including His-tag, SDS-PAGE
Zea mays
Purification (Commentary) (protein specific)
Commentary
Organism
-
Zea mays
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linolenate + O2
-
706251
Zea mays
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
the enzyme forms (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate and (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate in a 6:4 ratio. The R/S stereoconfiguration of the products is not determined
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 97000, recombinant enzyme including His-tag, SDS-PAGE
Zea mays
Other publictions for EC 1.13.11.B6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742627
Schiller
A dual positional specific li ...
Malus domestica
Hortic. Res.
2
15003-15015
2015
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-
1
-
7
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1
1
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4
-
4
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1
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2
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6
1
2
2
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2
2
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2
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8
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2
2
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4
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2
-
4
-
-
6
2
2
2
-
-
2
2
-
-
-
4
7
-
-
-
725658
Wennman
Secretion of two novel enzymes ...
Nakataea oryzae, Nakataea oryzae CBS 288.54
J. Lipid Res.
54
762-775
2013
-
-
1
-
2
1
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1
1
3
1
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5
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1
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1
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1
8
1
1
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1
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1
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2
1
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1
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1
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1
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1
8
1
1
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1
1
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-
-
-
2
2
-
1
1
725856
Jerneren
Linolenate 9R-dioxygenase and ...
Lasiodiplodia theobromae, Lasiodiplodia theobromae 2334, Lasiodiplodia theobromae CBS 117454, Lasiodiplodia theobromae CBS 122127
Lipids
47
65-73
2012
-
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1
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13
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6
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1
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17
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1
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1
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13
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1
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17
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1
-
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1
1
-
-
-
724736
Chechetkin
Oxidation of glycerolipids by ...
Zea mays
Chem. Phys. Lipids
164
216-220
2011
-
-
1
-
1
-
-
-
-
-
-
3
-
2
-
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12
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1
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1
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2
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3
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-
-
-
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-
12
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
702431
Palmieri-Thiers
A lipoxygenase with dual posit ...
Olea europaea
Biochim. Biophys. Acta
1791
339-346
2009
-
-
1
-
-
-
2
2
1
-
2
-
-
1
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1
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1
1
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2
1
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2
1
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2
1
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1
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2
2
2
1
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2
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1
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1
1
-
2
1
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-
-
2
1
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-
1
-
1
1
-
2
2
706122
Hornung
Identification of an amino aci ...
Momordica charantia
Phytochemistry
69
2774-2780
2008
-
-
1
-
2
-
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1
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1
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1
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1
1
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1
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1
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1
1
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1
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-
706252
Wang
A novel lipoxygenase gene from ...
Oryza sativa
Plant Mol. Biol.
66
401-414
2008
-
-
1
-
-
-
-
-
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-
1
1
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5
-
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3
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2
1
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1
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1
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1
1
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3
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2
1
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1
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2
1
1
2
-
-
674388
Cho
Biochemical characterization o ...
Zea mays
J. Biochem. Mol. Biol.
40
100-106
2007
-
-
-
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1
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1
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1
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1
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706289
Veronico
A novel lipoxygenase in pea ro ...
Pisum sativum
Plant Physiol.
141
1045-1055
2006
-
-
1
-
-
-
-
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1
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1
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1
1
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3
1
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1
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1
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1
1
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3
1
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-
1
1
1
1
-
-
703538
Santino
Biochemical and molecular char ...
Corylus avellana
Eur. J. Biochem.
270
4365-4375
2003
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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-
706251
Kim
Dual positional specificity an ...
Zea mays
Plant Mol. Biol.
52
1203-1213
2003
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
1
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-
396190
Hughes
Probing a novel potato lipoxyg ...
Solanum tuberosum
Biochem. J.
353
345-355
2001
-
-
1
-
3
-
-
1
-
-
-
-
-
7
-
-
1
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-
1
1
-
2
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1
1
1
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1
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3
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1
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1
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1
1
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2
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1
1
1
-
-
-
-
-
-
1
1
703535
Hughes
Mutagenesis and modelling of l ...
Pisum sativum
Eur. J. Biochem.
268
1030-1040
2001
-
-
1
-
8
-
-
1
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
1
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-
1
-
-
8
-
-
-
-
1
-
-
1
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-
1
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
706250
Burow
A peanut seed lipoxygenase res ...
Arachis hypogaea
Plant Mol. Biol.
42
689-701
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
1
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1
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-
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4
-
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1
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1
-
-
1
-
-
396184
Hughes
-
Characterization of authentic ...
Pisum sativum
Biochem. J.
333
33-43
1998
-
-
1
-
-
-
-
3
-
-
4
-
-
2
-
-
1
2
-
1
4
-
2
1
-
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-
3
3
-
-
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-
2
-
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-
-
-
3
-
-
4
-
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-
-
2
-
2
4
-
2
2
-
-
-
3
3
-
-
-
-
-
-
-
3
3
706645
Skrzypczak-Jankun
Structure of soybean lipoxygen ...
Glycine max
Proteins
29
15-31
1997
-
-
-
1
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-
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-
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
702394
Gardner
Soybean lipoxygenase-1 enzymic ...
Glycine max
Biochim. Biophys. Acta
1001
274-281
1989
-
-
-
-
-
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1
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1
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1
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