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Literature summary for 1.13.11.88 extracted from

  • Ryu, J.Y.; Seo, J.; Park, S.; Ahn, J.H.; Chong, Y.; Sadowsky, M.J.; Hur, H.G.
    Characterization of an isoeugenol monooxygenase (Iem) from Pseudomonas nitroreducens Jin1 that transforms isoeugenol to vanillin (2013), Biosci. Biotechnol. Biochem., 77, 289-294 .
    View publication on PubMed
Search for more literature for 1.13.11.88

Activating Compound

Activating Compound Comment Organism Structure
additional information not inhibitory or activating at 1 mM: Mg(II), Mn(II), Mo(II), NADH, FAD, or ascorbic acid, EDTA, 1,10-phenanthroline, and Tiron Pseudomonas nitroreducens

Cloned(Commentary)

Cloned (Comment) Organism
expression in ERscherichia coli Pseudomonas nitroreducens

Protein Variants

Protein Variants Comment Organism
H167A mutation of conserved His residue, involved in binding of Fe2+. Loss of catalytic activity Pseudomonas nitroreducens
H205A mutation of conserved His residue, 6.8% residual catalytic activity Pseudomonas nitroreducens
H218A mutation of conserved His residue, involved in binding of Fe2+. Loss of catalytic activity Pseudomonas nitroreducens
H282A mutation of conserved His residue, involved in binding of Fe2+. Loss of catalytic activity Pseudomonas nitroreducens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 40% of initial activity Pseudomonas nitroreducens
additional information not inhibitory or activating at 1 mM: Mg(II), Mn(II), Mo(II), NADH, FAD, or ascorbic acid, EDTA, 1,10-phenanthroline, and Tiron Pseudomonas nitroreducens
Ni2+ 1 mM, 49% of initial activity Pseudomonas nitroreducens
Zn2+ 1 mM, 35% of initial activity Pseudomonas nitroreducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 isoeugenol pH 8.0, 30°C Pseudomonas nitroreducens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ presence of 0.7 mol iron per mol of enzyme Pseudomonas nitroreducens
Fe3+ 1 mM, 114% of initial activity Pseudomonas nitroreducens

Organism

Organism UniProt Comment Textmining
Pseudomonas nitroreducens C3VA26
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
 pH 8.0, 30°C Pseudomonas nitroreducens

Storage Stability

Storage Stability Organism
-70°C, 20mM Tris/HCl, 10% glycerol, and 1mM dithiothreitol, pH 8.0, stable for several months Pseudomonas nitroreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isoeugenol + O2
-
 Pseudomonas nitroreducens vanillin + acetaldehyde
-
 ?
additional information oxidative cleavage of isoeugenol by Iem is catalyzed via a monooxygenation reaction, and incorporation of the oxygen atom from O2 into vanillin is preferred over incorporation from water. Iem exhibits no activity toward any other of the phenylpropanoid and styrene compounds tested Pseudomonas nitroreducens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Pseudomonas nitroreducens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas nitroreducens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 stable for 30 min Pseudomonas nitroreducens
30
-
 30 min, 7% loss of activity Pseudomonas nitroreducens
50
-
 30 min, complete loss of activity Pseudomonas nitroreducens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 isoeugenol pH 8.0, 30°C Pseudomonas nitroreducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas nitroreducens