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Literature summary for 1.13.11.87 extracted from

  • Birke, J.; Röther, W.; Jendrossek, D.
    Latex clearing protein (Lcp) of Streptomyces sp. strain K30 is a b-type cytochrome and differs from rubber oxygenase A (RoxA) in its biophysical properties (2015), Appl. Environ. Microbiol., 81, 3793-3799 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp. K30

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 20% inhibition Streptomyces sp. K30
diethyldithiocarbamate 1 mM, 56% inhibition Streptomyces sp. K30
Diethylthiocarbamate 1 mM, 56% inhibition Streptomyces sp. K30
Dithionite 1 mM, 15% inhibition Streptomyces sp. K30
ethyl xanthogenate 1 mM, 7% inhibition Streptomyces sp. K30
imidazole 1 mM, 48% inhibition Streptomyces sp. K30

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ purified Lcp contains an oxidized heme-Fe3+ Streptomyces sp. K30

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
SDS-PAGE Streptomyces sp. K30

Organism

Organism UniProt Comment Textmining
Streptomyces sp. K30 Q3L8N0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp. K30

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
23°C. pH 7.0 Streptomyces sp. K30
4.6
-
37°C, pH 7.0 Streptomyces sp. K30

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + O2
-
Streptomyces sp. K30 ?
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Streptomyces sp. K30

Synonyms

Synonyms Comment Organism
latex clearing protein
-
Streptomyces sp. K30
Lcp
-
Streptomyces sp. K30

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61.5
-
melting point Streptomyces sp. K30

Cofactor

Cofactor Comment Organism Structure
heme the enzyme is a member of the b-type cytochrome family Streptomyces sp. K30