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Literature summary for 1.13.11.85 extracted from

  • Birke, J.; Roether, W.; Jendrossek, D.
    RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp) (2017), Appl. Environ. Microbiol., 83, e00721-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Xanthomonas sp. 35Y

Inhibitors

Inhibitors Comment Organism Structure
CO carbon monoxide strongly inhibits the polyisoprene cleavage reaction of RoxAXsp Xanthomonas sp. 35Y

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71500
-
mature protein Xanthomonas sp. 35Y
74700
-
preprotein Xanthomonas sp. 35Y

Organism

Organism UniProt Comment Textmining
Xanthomonas sp. 35Y A0A1Y0DKQ1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the preprotein has a MW of 74700 Da, the mature protein has MW of 71500 Da Xanthomonas sp. 35Y

Purification (Commentary)

Purification (Comment) Organism
-
Xanthomonas sp. 35Y

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
30°C, pH not specified in the publication Xanthomonas sp. 35Y
2.6
-
37°C, pH not specified in the publication Xanthomonas sp. 35Y

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(cis-1,4-isoprene) + O2
-
Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
?

Synonyms

Synonyms Comment Organism
RoxB
-
Xanthomonas sp. 35Y

Cofactor

Cofactor Comment Organism Structure
heme c-type diheme protein Xanthomonas sp. 35Y

Expression

Organism Comment Expression
Xanthomonas sp. 35Y expression of the roxA gene is upregulated during growth on polyisoprene up