Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Xanthomonas sp. 35Y | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-1,4-polyisoprene + O2 | Xanthomonas sp. 35Y | - |
(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas sp. 35Y | Q7X0P3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthomonas sp. 35Y |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-1,4-polyisoprene + O2 | - |
Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
cis-1,4-polyisoprene + O2 | exo-type cleavage mechanism. RoxA is able to cleave isolated latex-clearing protein-derived oligo-isoprenoid molecules to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal groups | Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
roxA | - |
Xanthomonas sp. 35Y |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Xanthomonas sp. 35Y |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 30 | at 30°C, the specific activity of RoxA is almost double as high as at 23°C | Xanthomonas sp. 35Y |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
no significant reduction in activity after 22 h | Xanthomonas sp. 35Y |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | in potassium phosphate buffer or in HEPES buffer. Other buffers (Tris, carbonate-ammonium acetate, and glycylglycine) result in poor activities | Xanthomonas sp. 35Y |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH values below pH 6 or above 8.5 result in only poor activities | Xanthomonas sp. 35Y |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Xanthomonas sp. 35Y |