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Literature summary for 1.13.11.8 extracted from
- Protocatechuate 4,5-dioxygenase from Comamonas testosteroni T-2: biochemical and molecular properties of a new subgroup within class III of extradiol dioxygenases (2005), Arch. Microbiol., 183, 130-139.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
protocatechuate | 30°C, pH 7.5 | Comamonas testosteroni |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
alpha,beta, 4 * 18000 + 4 * 31000, SDS-PAGE | Comamonas testosteroni |
| 31000 | - |
alpha,beta, 4 * 18000 + 4 * 31000, SDS-PAGE | Comamonas testosteroni |
| 180000 | - |
gel filtration | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protocatechuate + O2 | Comamonas testosteroni | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
| protocatechuate + O2 | Comamonas testosteroni T-2 | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | Q8RNX9 | beta-subunit; T-2 | - |
| Comamonas testosteroni | Q8RNY0 | alpha-subunit; T-2 | - |
| Comamonas testosteroni T-2 | Q8RNX9 | beta-subunit; T-2 | - |
| Comamonas testosteroni T-2 | Q8RNY0 | alpha-subunit; T-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| heat treatment, DEAE-Sepharose, superdex 200, Resource Q | Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
- |
Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gallate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| gallate + O2 | - |
Comamonas testosteroni T-2 | ? | - |
? | |
| protocatechuate + O2 | - |
Comamonas testosteroni | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
| protocatechuate + O2 | - |
Comamonas testosteroni T-2 | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | alpha,beta, 4 * 18000 + 4 * 31000, SDS-PAGE | Comamonas testosteroni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PmdAB | - |
Comamonas testosteroni |
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Release 2023.1 (January 2023)
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