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show all sequences of 1.13.11.77

Isolation and characterization of a lipoxygenase from Pseudomonas 42A2 responsible for the biotransformation of oleic acid into (S)-(E)-10-hydroxy-8-octadecenoic acid

Busquets, M.; Deroncel, V.; Vidal-Mas, J.; Rodrguez, E.; Guerrero, A.; Manresa, A.; Antonie van Leeuwenhoek 85, 129-139 (2004)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ba2+
complete inhibition at 1 mM
Pseudomonas sp.
EDTA
99% inhibition at 5 mM
Pseudomonas sp.
Fe3+
complete inhibition at 1 mM
Pseudomonas sp.
Mg2+
concentrations of Mg2+ higher than 1.25 mM inhibit activity strongly (90% of activity lost at 1.75 mM)
Pseudomonas sp.
additional information
not inhibited by 5 mM KCN
Pseudomonas sp.
oleate
at concentrations of oleate higher than 2.1 mM, the enzyme activity is inhibited by excess of substrate
Pseudomonas sp.
Zn2+
complete inhibition at 1 mM
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.66
-
linoleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
0.73
-
alpha-linolenate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
0.74
-
oleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Pseudomonas sp.
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
the enzyme contains 0.55 mol of Fe2+ per mol of protein
Pseudomonas sp.
Mg2+
the presence of 1 mM Mg2+ increases enzyme activity 1.5fold
Pseudomonas sp.
Mn2+
the enzyme contains 25 mmol Mn2+ per mol of protein
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
1 * 45000, SDS-PAGE
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleate + O2
Pseudomonas sp.
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
linoleate + O2
Pseudomonas sp. 42A2
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
oleate + O2
Pseudomonas sp.
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
oleate + O2
Pseudomonas sp. 42A2
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 42A2
-
-
-
Purification (Commentary)
Commentary
Organism
Q Sepharose column chromatography and Macro-Prep SE 100/40 gel filtration
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
4C, 1 week, no loss of activity (thawing and freezing decreases enzyme activity by 30%)
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp. 42A2
?
-
-
-
?
cis-12-octadecenoate + O2
8.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
linoleate + O2
100% activity
723962
Pseudomonas sp.
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
linoleate + O2
100% activity
723962
Pseudomonas sp. 42A2
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp.
?
-
-
-
-
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp. 42A2
?
-
-
-
-
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
petroselinoate + O2
26.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
triolein + O2
0.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
30
-
Pseudomonas sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
70
44% of activity still remains at 55C. The enzyme is stable up to 45 C, but sharply decreases after 50C. Activity is completely lost at 70C
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ba2+
complete inhibition at 1 mM
Pseudomonas sp.
EDTA
99% inhibition at 5 mM
Pseudomonas sp.
Fe3+
complete inhibition at 1 mM
Pseudomonas sp.
Mg2+
concentrations of Mg2+ higher than 1.25 mM inhibit activity strongly (90% of activity lost at 1.75 mM)
Pseudomonas sp.
additional information
not inhibited by 5 mM KCN
Pseudomonas sp.
oleate
at concentrations of oleate higher than 2.1 mM, the enzyme activity is inhibited by excess of substrate
Pseudomonas sp.
Zn2+
complete inhibition at 1 mM
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.66
-
linoleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
0.73
-
alpha-linolenate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
0.74
-
oleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Pseudomonas sp.
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
the enzyme contains 0.55 mol of Fe2+ per mol of protein
Pseudomonas sp.
Mg2+
the presence of 1 mM Mg2+ increases enzyme activity 1.5fold
Pseudomonas sp.
Mn2+
the enzyme contains 25 mmol Mn2+ per mol of protein
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
1 * 45000, SDS-PAGE
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleate + O2
Pseudomonas sp.
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
linoleate + O2
Pseudomonas sp. 42A2
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
oleate + O2
Pseudomonas sp.
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
oleate + O2
Pseudomonas sp. 42A2
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Q Sepharose column chromatography and Macro-Prep SE 100/40 gel filtration
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
4C, 1 week, no loss of activity (thawing and freezing decreases enzyme activity by 30%)
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp. 42A2
?
-
-
-
?
cis-12-octadecenoate + O2
8.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
linoleate + O2
100% activity
723962
Pseudomonas sp.
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
linoleate + O2
100% activity
723962
Pseudomonas sp. 42A2
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp.
?
-
-
-
-
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp. 42A2
?
-
-
-
-
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
petroselinoate + O2
26.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
triolein + O2
0.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
30
-
Pseudomonas sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
70
44% of activity still remains at 55C. The enzyme is stable up to 45 C, but sharply decreases after 50C. Activity is completely lost at 70C
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Pseudomonas sp.
Other publictions for EC 1.13.11.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744582
Kim
Production of 10S-hydroxy-8(E ...
Nostoc punctiforme
Biotechnol. Lett.
39
133-139
2017
1
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1
-
1
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3
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4
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1
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4
1
1
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1
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3
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3
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1
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3
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4
1
1
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1
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-
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744396
Mashhadi
Robust inhibitory effects of ...
Nostoc punctiforme
Biochim. Biophys. Acta
1851
1346-1352
2015
-
-
-
-
-
-
11
-
-
-
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1
-
3
-
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1
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11
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1
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1
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745297
Brash
An ancient relative of cycloo ...
Nostoc punctiforme
J. Biol. Chem.
289
13101-13111
2014
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1
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3
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3
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6
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1
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6
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3
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1
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3
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3
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1
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6
-
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-
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3
-
-
-
-
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3
3
-
3
3
725415
Martinez
Biochemical characterization o ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa 42A2
J. Biol. Chem.
285
9339-9345
2010
-
-
-
-
-
-
-
-
-
-
1
-
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4
-
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2
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1
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2
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723962
Busquets
Isolation and characterization ...
Pseudomonas sp., Pseudomonas sp. 42A2
Antonie van Leeuwenhoek
85
129-139
2004
-
-
-
-
-
-
7
3
1
3
1
4
-
2
-
-
1
-
-
-
-
1
13
1
1
-
1
-
1
-
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7
-
3
1
3
1
4
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1
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1
13
1
1
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1
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1
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