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Literature summary for 1.13.11.76 extracted from

  • Wu, J.F.; Sun, C.W.; Jiang, C.Y.; Liu, Z.P.; Liu, S.J.
    A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli (2005), Arch. Microbiol., 183, 1-8.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, 79% residual activity Comamonas testosteroni
Cd2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni
Co2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni
Cu2+ 2 mM, 2.9% residual activity Comamonas testosteroni
EDTA 2 mM, 50% residual activity Comamonas testosteroni
Fe2+ 2 mM, 57% residual activity. Not inhibitory in presence of ascorbate Comamonas testosteroni
H2O2 2 mM, complete loss of activity Comamonas testosteroni
Mg2+ 2 mM, 91% residual activity Comamonas testosteroni
Mn2+ 2 mM, 31% residual activity Comamonas testosteroni
Ni2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni
Zn2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00089
-
2-Aminophenol pH 8.0, 22°C Comamonas testosteroni
0.00077
-
2-amino-5-chlorophenol pH 8.0, 22°C Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
gel filtration Comamonas testosteroni
38000
-
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE Comamonas testosteroni
33000
-
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni Q6J1Z5 subunit alpha
-

Purification (Commentary)

Purification (Comment) Organism
-
Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.7
-
pH 8.0, 22°C Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol Comamonas testosteroni ?
-
?
catechol + O2 5% of the activity with 2-aminophenol Comamonas testosteroni ?
-
?
protocatechuate + O2 33% of the activity with 2-aminophenol Comamonas testosteroni ?
-
?
2-amino-5-chlorophenol + O2 68% of the activity with 2-aminophenol Comamonas testosteroni 2-amino-5-chloromuconate 6-semialdehyde
-
?
2-aminophenol + O2
-
Comamonas testosteroni ?
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE Comamonas testosteroni

Synonyms

Synonyms Comment Organism
CnbcA
-
Comamonas testosteroni