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Literature summary for 1.13.11.68 extracted from

  • Harrison, P.J.; Newgas, S.A.; Descombes, F.; Shepherd, S.A.; Thompson, A.J.; Bugg, T.D.
    Biochemical characterization and selective inhibition of beta-carotene cis-trans isomerase D27 and carotenoid cleavage dioxygenase CCD8 on the strigolactone biosynthetic pathway (2015), FEBS J., 282, 3986-4000 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ccd7, recombinant expression of GST-tagged AtCCD7 in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by hydroxamic acids Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
9-cis-beta-carotene + O2 Arabidopsis thaliana
-
9-cis-10'-apo-beta-carotenal + beta-ionone
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q7XJM2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged AtCCD7 from Escherichia coli by glutathione affinity chromatographyy Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9-cis-beta-carotene + O2
-
Arabidopsis thaliana 9-cis-10'-apo-beta-carotenal + beta-ionone
-
?
9-cis-beta-carotene + O2 AtCCD7 preferentially cleaves 9-cis-beta-carotene Arabidopsis thaliana 9-cis-10'-apo-beta-carotenal + beta-ionone
-
?

Synonyms

Synonyms Comment Organism
carotenoid cleavage dioxygenase
-
Arabidopsis thaliana
CCD7
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene, strigolactone biosynthesis pathway from all-trans-beta-carotene to ent-2'-epi-5-deoxystrigol Arabidopsis thaliana
additional information the biochemical basis of the shoot branching phenotype is not due to inhibition of enzyme CCD7, but of enzyme CCD8, EC 1.13.11.70 Arabidopsis thaliana
physiological function biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene Arabidopsis thaliana