Cloned (Comment) | Organism |
---|---|
gene ccd7, recombinant expression of GST-tagged AtCCD7 in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by hydroxamic acids | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-cis-beta-carotene + O2 | Arabidopsis thaliana | - |
9-cis-10'-apo-beta-carotenal + beta-ionone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q7XJM2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged AtCCD7 from Escherichia coli by glutathione affinity chromatographyy | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-cis-beta-carotene + O2 | - |
Arabidopsis thaliana | 9-cis-10'-apo-beta-carotenal + beta-ionone | - |
? | |
9-cis-beta-carotene + O2 | AtCCD7 preferentially cleaves 9-cis-beta-carotene | Arabidopsis thaliana | 9-cis-10'-apo-beta-carotenal + beta-ionone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carotenoid cleavage dioxygenase | - |
Arabidopsis thaliana |
CCD7 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene, strigolactone biosynthesis pathway from all-trans-beta-carotene to ent-2'-epi-5-deoxystrigol | Arabidopsis thaliana |
additional information | the biochemical basis of the shoot branching phenotype is not due to inhibition of enzyme CCD7, but of enzyme CCD8, EC 1.13.11.70 | Arabidopsis thaliana |
physiological function | biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene | Arabidopsis thaliana |