Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | uncultured marine bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenanthroline | 2% residual activity | uncultured marine bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
beta-cryptoxanthin | pH 8.0, 40°C | uncultured marine bacterium | |
0.037 | - |
beta-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
0.147 | - |
beta-apo-4'-carotenal | pH 8.0, 40°C | uncultured marine bacterium | |
0.169 | - |
alpha-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
0.382 | - |
gamma-carotene | pH 8.0, 40°C | uncultured marine bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | addition of 10 microM Fe2+ after the removal of all metal ions induces a 54% recovery of the active form of the enzyme. Optimal concentration is 10 microM, regardless of phenanthroline treatment | uncultured marine bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | uncultured marine bacterium |
64000 | - |
gel fitlration | uncultured marine bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured marine bacterium | Q4PNI0 | - |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant protein | uncultured marine bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-carotene + O2 | - |
uncultured marine bacterium | ? | - |
? | |
beta-apo-4'-carotenal + O2 | - |
uncultured marine bacterium | ? | - |
? | |
beta-carotene + O2 | - |
uncultured marine bacterium | 2 all-trans-retinal | the oxygen atom of retinal originates from molecular oxygen | ? | |
beta-cryptoxanthin + O2 | - |
uncultured marine bacterium | retinal + (3R)-3-hydroxyretinal | - |
? | |
gamma-carotene + O2 | - |
uncultured marine bacterium | ? | - |
? | |
additional information | no substrate: beta-apo-8'-carotenal, beta-apo-12'-carotenal, lutein, zeaxanthin, or lycopene, suggesting that the presence of one unsubstituted-ionone ring in a substrate with a molecular weight greater than C35 seems to be essential for enzyme activity | uncultured marine bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | uncultured marine bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
uncultured marine bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
15% of maximum activity | uncultured marine bacterium |
50 | - |
66% of maximum activity | uncultured marine bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
half-life 17.6 min | uncultured marine bacterium |
40 | - |
half-life 15 min | uncultured marine bacterium |
45 | - |
half-life 12.5 min | uncultured marine bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
gamma-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
0.001 | - |
alpha-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
0.01 | - |
beta-apo-4'-carotenal | pH 8.0, 40°C | uncultured marine bacterium | |
0.013 | - |
beta-cryptoxanthin | pH 8.0, 40°C | uncultured marine bacterium | |
0.037 | - |
beta-carotene | pH 8.0, 40°C | uncultured marine bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
uncultured marine bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
gamma-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
0.06 | - |
alpha-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
0.07 | - |
beta-apo-4'-carotenal | pH 8.0, 40°C | uncultured marine bacterium | |
0.47 | - |
beta-cryptoxanthin | pH 8.0, 40°C | uncultured marine bacterium | |
1.62 | - |
beta-carotene | pH 8.0, 40°C | uncultured marine bacterium |