Application | Comment | Organism |
---|---|---|
synthesis | production of retinal from beta-carotene using recombinant enzyme. The optimum pH, temperature, substrate and detergent concentrations, and enzyme amount for effective retinal production are 9.0, 37°C, 200 mg per ml beta-carotene, 5% w/v Tween 40, and 0.2 U per ml enzyme, respectively. Under optimum conditions, the recombinant enzyme produces 72 mg per ml retinal in a 15 h reaction time, with a conversion yield of 36% w/w | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64686 | - |
x * 64686, calculated, x * 65000, SDS-PAGE | Mus musculus |
65000 | - |
x * 64686, calculated, x * 65000, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9JJS6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.51 | - |
pH 9.0, 36°C | Mus musculus |
Subunits | Comment | Organism |
---|---|---|
? | x * 64686, calculated, x * 65000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mus musculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
22% of maximum activity | Mus musculus |
45 | - |
14% of maximum activity | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
70% of maximum activity | Mus musculus |
10 | - |
70% of maximum activity | Mus musculus |