Cloned (Comment) | Organism |
---|---|
gene GLRG_10013, phylogenetic analysis | Colletotrichum graminicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate | Colletotrichum graminicola | - |
(7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate | - |
r | |
additional information | Colletotrichum graminicola | the dioxygenase-cytochrome P450 homologue oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | ? | - |
? | |
additional information | Colletotrichum graminicola M1.001 | the dioxygenase-cytochrome P450 homologue oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | ? | - |
? | |
additional information | Colletotrichum graminicola M1.001 / M2 / FGSC 10212 | the dioxygenase-cytochrome P450 homologue oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | ? | - |
? | |
oleate + O2 | Colletotrichum graminicola | - |
(9Z)-7,8-dihydroxyoctadeca-9-enoic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colletotrichum graminicola | E3QVI1 | - |
- |
Colletotrichum graminicola M1.001 | E3QVI1 | - |
- |
Colletotrichum graminicola M1.001 / M2 / FGSC 10212 | E3QVI1 | - |
- |
Fusarium oxysporum | F9FU71 | - |
- |
Fusarium oxysporum Fo5176 | F9FU71 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-8-hydroperoxy-(9Z,12Z)-octadecadienoate + O2 | - |
Colletotrichum graminicola | (8R,11R)-8,11-dihydroperoxy-(9Z,12Z)-octadecadienoate + (7R,8R)-7,8-dihydroperoxy-(9Z,12Z)-octadecadienoate | - |
? | |
(R)-8-hydroperoxy-(9Z,12Z)-octadecadienoate + O2 | - |
Colletotrichum graminicola M1.001 | (8R,11R)-8,11-dihydroperoxy-(9Z,12Z)-octadecadienoate + (7R,8R)-7,8-dihydroperoxy-(9Z,12Z)-octadecadienoate | - |
? | |
(R)-8-hydroperoxy-(9Z,12Z)-octadecadienoate + O2 | - |
Colletotrichum graminicola M1.001 / M2 / FGSC 10212 | (8R,11R)-8,11-dihydroperoxy-(9Z,12Z)-octadecadienoate + (7R,8R)-7,8-dihydroperoxy-(9Z,12Z)-octadecadienoate | - |
? | |
alpha-linolenic acid + O2 | - |
Colletotrichum graminicola | ? | - |
? | |
alpha-linolenic acid + O2 | - |
Colletotrichum graminicola M1.001 | ? | - |
? | |
alpha-linolenic acid + O2 | - |
Colletotrichum graminicola M1.001 / M2 / FGSC 10212 | ? | - |
? | |
arachidonic acid + O2 | - |
Fusarium oxysporum | (R)-10-hydroxyarachidonic acid | - |
? | |
arachidonic acid + O2 | - |
Fusarium oxysporum Fo5176 | (R)-10-hydroxyarachidonic acid | - |
? | |
docosapentaenoic acid + O2 | - |
Fusarium oxysporum | (R)-10-hydroperoxy-docosapentaenoic acid | - |
? | |
docosapentaenoic acid + O2 | - |
Fusarium oxysporum Fo5176 | (R)-10-hydroperoxy-docosapentaenoic acid | - |
? | |
gamma-linolenic acid + O2 | - |
Fusarium oxysporum | (R)-8-hydroxy-gamma-linolenic acid | - |
? | |
gamma-linolenic acid + O2 | - |
Fusarium oxysporum Fo5176 | (R)-8-hydroxy-gamma-linolenic acid | - |
? | |
linoleate | - |
Colletotrichum graminicola | (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate | - |
r | |
linoleate + O2 | - |
Colletotrichum graminicola | (R)-8-hydroperoxy-(9Z,12Z)-octadecadienoate + (8R,11R)-8,11-dihydroperoxy-(9Z,12Z)-octadecadienoate + (7R,8R)-7,8-dihydroperoxy-(9Z,12Z)-octadecadienoate | - |
? | |
linoleate + O2 | - |
Fusarium oxysporum | (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid | - |
? | |
linoleate + O2 | - |
Fusarium oxysporum Fo5176 | (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid | - |
? | |
additional information | enzyme metabolizes fatty acids to hydroperoxides with broad substrate specificity. Products show R configuration at the (n-10) positions. Fatty acids of the n-3 series are oxidized less efficiently and often to hydroperoxides with an R configuration at both (n-10) and (n-7) positions | Fusarium oxysporum | ? | - |
? | |
additional information | enzyme oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases, but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | Colletotrichum graminicola | ? | - |
? | |
additional information | no substrate: gamma-linolenoate | Colletotrichum graminicola | ? | - |
? | |
additional information | the dioxygenase-cytochrome P450 homologue oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | Colletotrichum graminicola | ? | - |
? | |
additional information | enzyme oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases, but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | Colletotrichum graminicola M1.001 | ? | - |
? | |
additional information | no substrate: gamma-linolenoate | Colletotrichum graminicola M1.001 | ? | - |
? | |
additional information | the dioxygenase-cytochrome P450 homologue oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | Colletotrichum graminicola M1.001 | ? | - |
? | |
additional information | enzyme oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases, but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | Colletotrichum graminicola M1.001 / M2 / FGSC 10212 | ? | - |
? | |
additional information | no substrate: gamma-linolenoate | Colletotrichum graminicola M1.001 / M2 / FGSC 10212 | ? | - |
? | |
additional information | the dioxygenase-cytochrome P450 homologue oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid | Colletotrichum graminicola M1.001 / M2 / FGSC 10212 | ? | - |
? | |
additional information | enzyme metabolizes fatty acids to hydroperoxides with broad substrate specificity. Products show R configuration at the (n-10) positions. Fatty acids of the n-3 series are oxidized less efficiently and often to hydroperoxides with an R configuration at both (n-10) and (n-7) positions | Fusarium oxysporum Fo5176 | ? | - |
? | |
oleate + O2 | - |
Colletotrichum graminicola | 8-hydroperoxyoctadecamonoenoate + 7,8-dihydroxyoctadecamonoenoate | - |
? | |
oleate + O2 | - |
Colletotrichum graminicola | (9Z)-7,8-dihydroxyoctadeca-9-enoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dioxygenase-cytochrome P450 | - |
Colletotrichum graminicola |
DOX-CYP | - |
Colletotrichum graminicola |
FOXB_09952 | - |
Fusarium oxysporum |
GLRG_10013 | - |
Colletotrichum graminicola |
linoleate 7,8-LDS | - |
Colletotrichum graminicola |
linoleate 8,11-LDS | - |
Colletotrichum graminicola |
linoleate diol synthase | - |
Colletotrichum graminicola |
orphan DOX-CYP | - |
Colletotrichum graminicola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
assay at | Fusarium oxysporum |
- |
- |
assay at | Colletotrichum graminicola |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
incubation at room temperature augments the non-enzymatic formation of epoxy alcohols | Fusarium oxysporum |
additional information | - |
incubation at room temperature augments the non-enzymatic formation of epoxy alcohols | Colletotrichum graminicola |
General Information | Comment | Organism |
---|---|---|
evolution | homologue dioxygenase-cytochrome P450 (DOX-CYP) is a fusion protein of the animal heme peroxidase (cyclooxygenase) superfamily. Phylogenetic tree of fungal fusion enzymes with homology to the animal heme peroxidase and CYP superfamilies, overview | Colletotrichum graminicola |
metabolism | fungal oxylipins can be formed by two subfamilies of cyclooxygenase-related DOX. The enzymatic activities of the DOX-CYP homologue of Colletotrichum graminicola and the DOX homologue of Fusarium oxysporum. The former oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid. The latter metabolizes fatty acids to hydroperoxides with broad substrate specificity. It oxidizes 20:4n-6 and 18:2n-6 to hydroperoxides with an R configuration at the (n-10) positions, and other n-6 fatty acids in the same way | Colletotrichum graminicola |