BRENDA - Enzyme Database
show all sequences of 1.13.11.60

Studies on linoleic acid 8R-dioxygenase and hydroperoxide isomerase of the fungus Gaeumannomyces graminis

Su, C.; Brodowsky, I.D.; Oliw, E.H.; Lipids 30, 43-50 (1995)

Data extracted from this reference:

General Stability
General Stability
Organism
8R-dioxygenase is unstable during purification. It can be stabilized by glutathione, glutathione peroxidase and ethylenediaminetetraacetic acid
Gaeumannomyces graminis
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-aminobenzamidine
1 mM, over 70% inhibition
Gaeumannomyces graminis
acetonitrile
10%, inactivation
Gaeumannomyces graminis
Emulgen 911
0.5%, inactivation
Gaeumannomyces graminis
glycine
2%
Gaeumannomyces graminis
ICI 230,487
0.03 mM, 58% inhibition
Gaeumannomyces graminis
Isopropanol
10%, inactivation
Gaeumannomyces graminis
linoleic acid
0.1 mM, partial inactivation, may indicate product inhibition
Gaeumannomyces graminis
additional information
sorbitol (0.75 M, 14%), glycerol (15%) and ethylene glycol (25%) do not inhibit the enzyme
Gaeumannomyces graminis
N-tosyl-L-phenylalanyl chloromethyl ketone
1 mM, over 70% inhibition
Gaeumannomyces graminis
paracetamol
0.03 mM, 44% inhibition
Gaeumannomyces graminis
polyoxyethylene-10 lauryl ether
0.5%, inactivation
Gaeumannomyces graminis
sodium deoxycholate
0.5%, inactivation
Gaeumannomyces graminis
Triton X-100
0.5%, inactivation
Gaeumannomyces graminis
Tween 20
0.5%, inactivation
Gaeumannomyces graminis
Tween 80
0.5%, inactivation
Gaeumannomyces graminis
Urea
1-4 M
Gaeumannomyces graminis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
linoleate + O2
Gaeumannomyces graminis
-
(8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Gaeumannomyces graminis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Gaeumannomyces graminis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
linoleate + O2
-
285249
Gaeumannomyces graminis
(8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
linoleic acid 8R-dioxygenase
-
Gaeumannomyces graminis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Gaeumannomyces graminis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Gaeumannomyces graminis
isoelectric focusing
6.3
6.1
General Stability (protein specific)
General Stability
Organism
8R-dioxygenase is unstable during purification. It can be stabilized by glutathione, glutathione peroxidase and ethylenediaminetetraacetic acid
Gaeumannomyces graminis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-aminobenzamidine
1 mM, over 70% inhibition
Gaeumannomyces graminis
acetonitrile
10%, inactivation
Gaeumannomyces graminis
Emulgen 911
0.5%, inactivation
Gaeumannomyces graminis
glycine
2%
Gaeumannomyces graminis
ICI 230,487
0.03 mM, 58% inhibition
Gaeumannomyces graminis
Isopropanol
10%, inactivation
Gaeumannomyces graminis
linoleic acid
0.1 mM, partial inactivation, may indicate product inhibition
Gaeumannomyces graminis
additional information
sorbitol (0.75 M, 14%), glycerol (15%) and ethylene glycol (25%) do not inhibit the enzyme
Gaeumannomyces graminis
N-tosyl-L-phenylalanyl chloromethyl ketone
1 mM, over 70% inhibition
Gaeumannomyces graminis
paracetamol
0.03 mM, 44% inhibition
Gaeumannomyces graminis
polyoxyethylene-10 lauryl ether
0.5%, inactivation
Gaeumannomyces graminis
sodium deoxycholate
0.5%, inactivation
Gaeumannomyces graminis
Triton X-100
0.5%, inactivation
Gaeumannomyces graminis
Tween 20
0.5%, inactivation
Gaeumannomyces graminis
Tween 80
0.5%, inactivation
Gaeumannomyces graminis
Urea
1-4 M
Gaeumannomyces graminis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
linoleate + O2
Gaeumannomyces graminis
-
(8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Gaeumannomyces graminis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
linoleate + O2
-
285249
Gaeumannomyces graminis
(8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Gaeumannomyces graminis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Gaeumannomyces graminis
isoelectric focusing
6.3
6.1
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741712
Seo
Characterization of a recombi ...
Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961
Appl. Microbiol. Biotechnol.
100
3087-3099
2016
1
-
1
-
11
-
-
17
-
-
1
1
1
5
-
-
1
-
-
1
8
-
25
3
1
2
-
1
18
3
-
-
-
-
-
-
1
-
1
-
-
11
-
-
-
-
17
-
-
1
1
1
-
-
1
-
1
8
-
25
3
2
-
1
18
3
-
-
-
-
-
-
-
20
20
742730
Seo
Production of 7,8-dihydroxy u ...
Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961
J. Agric. Food Chem.
64
8555-8562
2016
1
1
1
-
1
-
-
-
-
-
-
2
-
6
-
-
1
-
-
1
-
-
14
-
3
1
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
743200
Sooman
Discovery of a novel linoleat ...
Colletotrichum graminicola, Colletotrichum graminicola M1.001, Colletotrichum graminicola M1.001 / M2 / FGSC 10212, Fusarium oxysporum, Fusarium oxysporum Fo5176
Lipids
50
1243-1252
2015
-
-
1
-
-
-
-
-
-
-
-
5
-
13
-
-
-
-
-
-
-
-
29
-
8
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
29
-
2
2
-
-
-
-
-
-
-
2
2
-
-
-
724132
Hoffmann
7,8- and 5,8-linoleate diol sy ...
Aspergillus fumigatus, Gaeumannomyces graminis
Arch. Biochem. Biophys.
539
87-91
2013
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
5
2
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
711024
Hoffmann
Expression of 5,8-LDS of Asper ...
Aspergillus fumigatus, Gaeumannomyces graminis
Arch. Biochem. Biophys.
506
216-222
2010
-
-
2
-
6
-
-
2
-
-
-
3
-
4
-
-
-
-
-
2
-
-
10
-
10
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
-
7
-
-
-
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2
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3
-
-
-
-
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
714317
Jernerén
Reaction mechanism of 5,8-lino ...
Aspergillus clavatus
Biochim. Biophys. Acta
1801
503-507
2010
-
-
-
-
-
-
-
-
-
-
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1
-
4
-
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2
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3
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1
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2
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704466
Brodhun
Identification of PpoA from As ...
Aspergillus nidulans
J. Biol. Chem.
284
11792-11805
2009
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1
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2
-
1
4
-
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2
1
-
1
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1
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1
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7
1
1
1
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4
1
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2
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1
2
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2
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1
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4
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2
1
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1
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1
-
7
1
1
-
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4
1
-
-
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-
-
-
4
4
704475
Garscha
Leucine/valine residues direct ...
Gaeumannomyces graminis
J. Biol. Chem.
284
13755-13765
2009
-
-
1
-
4
-
-
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-
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1
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2
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2
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2
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1
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4
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1
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2
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702018
Garscha
Pichia expression and mutagene ...
Gaeumannomyces graminis, Gaeumannomyces graminis var. avenae
Biochem. Biophys. Res. Commun.
373
579-583
2008
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-
1
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3
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-
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2
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4
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1
1
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4
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2
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1
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3
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2
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1
1
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4
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703700
Garscha
Critical amino acids for the 8 ...
Gaeumannomyces graminis
FEBS Lett.
582
3547-3551
2008
-
-
1
-
11
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
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-
1
-
3
-
-
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-
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-
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1
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1
1
-
11
-
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-
-
1
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285246
Hörnsten
Cloning of linoleate diol synt ...
Gaeumannomyces graminis
J. Biol. Chem.
274
28219-28224
1999
-
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1
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-
-
-
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1
1
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2
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1
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1
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1
1
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-
-
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1
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-
-
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-
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-
-
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285251
Oliw
Catalytic properties of linole ...
Gaeumannomyces graminis
Adv. Exp. Med. Biol.
469
679-685
1999
2
-
-
-
-
-
2
-
-
-
1
1
-
2
-
-
-
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1
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1
1
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1
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2
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1
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2
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1
1
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1
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1
1
-
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-
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-
-
285244
Su
A protein radical and ferryl i ...
Gaeumannomyces graminis
J. Biol. Chem.
273
20744-20751
1998
-
-
-
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-
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1
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2
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4
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1
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1
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5
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1
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2
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2
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2
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2
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2
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5
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-
-
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285243
Su
Purification and characterizat ...
Gaeumannomyces graminis
J. Biol. Chem.
271
14112-14118
1996
-
-
-
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2
2
2
-
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2
1
1
1
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1
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1
1
1
2
1
-
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-
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1
1
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1
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1
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-
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1
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2
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2
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2
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2
1
1
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1
-
1
1
1
2
1
-
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-
1
1
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-
1
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-
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-
285249
Su
Studies on linoleic acid 8R-di ...
Gaeumannomyces graminis
Lipids
30
43-50
1995
-
-
-
-
-
1
16
-
-
-
-
1
-
3
-
-
1
-
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1
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1
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1
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1
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1
-
16
-
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1
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1
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1
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1
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1
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285247
Brodowsky
BW A4C and other hydroxamic ac ...
Gaeumannomyces graminis
Eur. J. Pharmacol.
254
43-47
1994
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12
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1
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2
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1
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1
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1
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1
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6
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6
12
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1
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1
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1
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1
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1
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285248
Brodowsky
Linoleic acid 8R-dioxygenase a ...
Gaeumannomyces graminis
Ann. N. Y. Acad. Sci.
744
314-316
1994
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5
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1
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1
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2
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5
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5
5
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1
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1
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285250
Hamberg
Sequential oxygenation of lino ...
Gaeumannomyces graminis
Arch. Biochem. Biophys.
309
77-80
1994
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2
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1
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4
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285245
Brodowsky
A linoleic acid (8R)-dioxygena ...
Gaeumannomyces graminis
J. Biol. Chem.
267
14738-14745
1992
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