Data extracted from this reference:
General Stability
repeated freezing and thawing, particularly of dilute enzyme solutions, inactivates the enzyme
Gaeumannomyces graminis
the enzyme is quite unstable at the early steps of purification, but active fractions from anion-exchange chromatography can be kept for 1 week on ice with only a small loss of enzyme activity
Gaeumannomyces graminis
Inhibitors
additional information
not inhibited by 1 mM KCN. CO does not inhibit the enzyme
Gaeumannomyces graminis
N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid
i.e. BW A4C, more than 90% inhibition at 0.01 mM, about 50% inhibition at 0.001 mM
Gaeumannomyces graminis
KM Value [mM]
0.008
linoleate
pH 7.4 25°C
Gaeumannomyces graminis
0.03
O2
pH 7.4 25°C
Gaeumannomyces graminis
Molecular Weight [Da]
130000
4 * 130000, gel filtration
Gaeumannomyces graminis
520000
gel filtration
Gaeumannomyces graminis
Natural Substrates/ Products (Substrates)
linoleate + O2
Gaeumannomyces graminis
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
?
Organic Solvent Stability
urea
the enzyme can be incubated in 4 M urea for 1 h, followed by desalting, with only partial loss of enzyme activity
Gaeumannomyces graminis
Organism
Gaeumannomyces graminis
Q9UUS2
Purification (Commentary)
Source Tissue
mycelium
Gaeumannomyces graminis
Specific Activity [micromol/min/mg]
1.8
pH 7.4, 0°C
Gaeumannomyces graminis
Storage Stability
-80°C, stable
Gaeumannomyces graminis
Substrates and Products (Substrate)
linoleate + O2
285243
Gaeumannomyces graminis
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
?
linoleate + O2
H2O2 (up to 9 mM) does not support enzyme activity under anaerobic conditions
285243
Gaeumannomyces graminis
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
?
Subunits
tetramer
4 * 130000, gel filtration
Gaeumannomyces graminis
Turnover Number [1/s]
15
linoleate
pH 7.4, 0°C
Gaeumannomyces graminis
pH Optimum
7.2
7.4
Gaeumannomyces graminis
Cofactor
heme
the enzyme contains at least 2 mol of heme/mol of enzyme
Gaeumannomyces graminis
pI Value
Gaeumannomyces graminis
chromatofocusing
5.2
Cofactor (protein specific)
heme
the enzyme contains at least 2 mol of heme/mol of enzyme
Gaeumannomyces graminis
General Stability (protein specific)
repeated freezing and thawing, particularly of dilute enzyme solutions, inactivates the enzyme
Gaeumannomyces graminis
the enzyme is quite unstable at the early steps of purification, but active fractions from anion-exchange chromatography can be kept for 1 week on ice with only a small loss of enzyme activity
Gaeumannomyces graminis
Inhibitors (protein specific)
additional information
not inhibited by 1 mM KCN. CO does not inhibit the enzyme
Gaeumannomyces graminis
N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid
i.e. BW A4C, more than 90% inhibition at 0.01 mM, about 50% inhibition at 0.001 mM
Gaeumannomyces graminis
KM Value [mM] (protein specific)
0.008
linoleate
pH 7.4 25°C
Gaeumannomyces graminis
0.03
O2
pH 7.4 25°C
Gaeumannomyces graminis
Molecular Weight [Da] (protein specific)
130000
4 * 130000, gel filtration
Gaeumannomyces graminis
520000
gel filtration
Gaeumannomyces graminis
Natural Substrates/ Products (Substrates) (protein specific)
linoleate + O2
Gaeumannomyces graminis
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
?
Organic Solvent Stability (protein specific)
urea
the enzyme can be incubated in 4 M urea for 1 h, followed by desalting, with only partial loss of enzyme activity
Gaeumannomyces graminis
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
mycelium
Gaeumannomyces graminis
Specific Activity [micromol/min/mg] (protein specific)
1.8
pH 7.4, 0°C
Gaeumannomyces graminis
Storage Stability (protein specific)
-80°C, stable
Gaeumannomyces graminis
Substrates and Products (Substrate) (protein specific)
linoleate + O2
285243
Gaeumannomyces graminis
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
?
linoleate + O2
H2O2 (up to 9 mM) does not support enzyme activity under anaerobic conditions
285243
Gaeumannomyces graminis
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
?
Subunits (protein specific)
tetramer
4 * 130000, gel filtration
Gaeumannomyces graminis
Turnover Number [1/s] (protein specific)
15
linoleate
pH 7.4, 0°C
Gaeumannomyces graminis
pH Optimum (protein specific)
7.2
7.4
Gaeumannomyces graminis
pI Value (protein specific)
Gaeumannomyces graminis
chromatofocusing
5.2
Other publictions for EC 1.13.11.60
741712
Seo
Characterization of a recombi ...
Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961
Appl. Microbiol. Biotechnol.
100
3087-3099
2016
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-
2
-
11
-
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20
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2
1
1
7
-
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1
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9
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25
3
1
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20
5
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11
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20
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2
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3
4
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20
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-
-
-
-
-
-
20
20
742730
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Production of 7,8-dihydroxy u ...
Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961
J. Agric. Food Chem.
64
8555-8562
2016
1
1
2
-
1
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-
-
-
-
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2
-
11
-
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1
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1
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14
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3
1
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-
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1
-
-
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-
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1
1
2
-
-
1
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2
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1
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1
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-
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
743200
Sooman
Discovery of a novel linoleat ...
Colletotrichum graminicola, Fusarium oxysporum, Colletotrichum graminicola M1.001, Colletotrichum graminicola M1.001 / M2 / FGSC 10212, Fusarium oxysporum Fo5176
Lipids
50
1243-1252
2015
-
-
1
-
-
-
-
-
-
-
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5
-
18
-
-
-
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-
-
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29
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8
2
2
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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5
-
-
-
-
-
-
-
-
29
-
2
2
-
-
-
-
-
-
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2
2
-
-
-
724132
Hoffmann
7,8- and 5,8-linoleate diol sy ...
Aspergillus fumigatus, Gaeumannomyces graminis
Arch. Biochem. Biophys.
539
87-91
2013
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2
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7
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-
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5
2
4
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2
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7
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-
5
2
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-
-
-
-
-
-
-
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2
2
-
-
-
711024
Hoffmann
Expression of 5,8-LDS of Asper ...
Aspergillus fumigatus, Gaeumannomyces graminis
Arch. Biochem. Biophys.
506
216-222
2010
-
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2
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7
-
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2
-
-
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3
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6
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-
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-
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2
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-
10
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10
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
-
7
-
-
-
-
2
-
-
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3
-
-
-
-
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
714317
Jernerén
Reaction mechanism of 5,8-lino ...
Aspergillus clavatus
Biochim. Biophys. Acta
1801
503-507
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
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-
-
-
-
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2
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3
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1
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-
-
-
2
-
-
-
-
-
-
-
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-
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-
-
-
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704466
Brodhun
Identification of PpoA from As ...
Aspergillus nidulans
J. Biol. Chem.
284
11792-11805
2009
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-
1
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2
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1
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
7
1
1
1
-
-
4
1
-
-
2
-
-
-
-
-
1
2
-
2
-
-
1
-
4
-
-
2
1
-
-
-
1
-
-
1
-
7
1
1
-
-
4
1
-
-
-
-
-
-
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4
4
704475
Garscha
Leucine/valine residues direct ...
Gaeumannomyces graminis
J. Biol. Chem.
284
13755-13765
2009
-
-
1
-
4
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702018
Garscha
Pichia expression and mutagene ...
Gaeumannomyces graminis, Gaeumannomyces graminis var. avenae
Biochem. Biophys. Res. Commun.
373
579-583
2008
-
-
1
-
3
-
-
-
-
-
-
2
-
6
-
1
1
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703700
Garscha
Critical amino acids for the 8 ...
Gaeumannomyces graminis
FEBS Lett.
582
3547-3551
2008
-
-
1
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
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285246
Hörnsten
Cloning of linoleate diol synt ...
Gaeumannomyces graminis
J. Biol. Chem.
274
28219-28224
1999
-
-
1
-
-
-
-
-
-
1
1
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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285251
Oliw
Catalytic properties of linole ...
Gaeumannomyces graminis
Adv. Exp. Med. Biol.
469
679-685
1999
2
-
-
-
-
-
2
-
-
-
1
1
-
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
2
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285244
Su
A protein radical and ferryl i ...
Gaeumannomyces graminis
J. Biol. Chem.
273
20744-20751
1998
-
-
-
-
-
-
1
-
-
-
-
2
-
5
-
-
2
-
-
2
-
-
5
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
2
-
2
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285243
Su
Purification and characterizat ...
Gaeumannomyces graminis
J. Biol. Chem.
271
14112-14118
1996
-
-
-
-
-
2
2
2
-
-
2
1
1
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
1
1
-
-
1
-
1
-
-
-
-
1
-
-
2
-
2
-
2
-
-
2
1
1
-
-
1
-
1
1
1
2
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
285249
Su
Studies on linoleic acid 8R-di ...
Gaeumannomyces graminis
Lipids
30
43-50
1995
-
-
-
-
-
1
16
-
-
-
-
1
-
6
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
16
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
285247
Brodowsky
BW A4C and other hydroxamic ac ...
Gaeumannomyces graminis
Eur. J. Pharmacol.
254
43-47
1994
-
-
-
-
-
-
12
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
6
-
-
-
-
-
-
-
6
12
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
285248
Brodowsky
Linoleic acid 8R-dioxygenase a ...
Gaeumannomyces graminis
Ann. N. Y. Acad. Sci.
744
314-316
1994
-
-
-
-
-
-
5
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
5
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285250
Hamberg
Sequential oxygenation of lino ...
Gaeumannomyces graminis
Arch. Biochem. Biophys.
309
77-80
1994
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
2
-
-
-
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-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285245
Brodowsky
A linoleic acid (8R)-dioxygena ...
Gaeumannomyces graminis
J. Biol. Chem.
267
14738-14745
1992
-
-
-
-
-
-
-
-
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1
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1
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-
-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
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-
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-
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-
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-
1
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-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-