BRENDA - Reference to 1.13.11.60; Id = 285243

repeated freezing and thawing, particularly of dilute enzyme solutions, inactivates the enzyme

Gaeumannomyces graminis

the enzyme is quite unstable at the early steps of purification, but active fractions from anion-exchange chromatography can be kept for 1 week on ice with only a small loss of enzyme activity

Gaeumannomyces graminis

additional information

not inhibited by 1 mM KCN. CO does not inhibit the enzyme

Gaeumannomyces graminis

N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid

i.e. BW A4C, more than 90% inhibition at 0.01 mM, about 50% inhibition at 0.001 mM

Gaeumannomyces graminis

0.008

-

linoleate

pH 7.4 25°C

Gaeumannomyces graminis

0.03

-

O2

pH 7.4 25°C

Gaeumannomyces graminis

130000

-

4 * 130000, gel filtration

Gaeumannomyces graminis

520000

-

gel filtration

Gaeumannomyces graminis

linoleate + O2

Gaeumannomyces graminis

-

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

urea

the enzyme can be incubated in 4 M urea for 1 h, followed by desalting, with only partial loss of enzyme activity

Gaeumannomyces graminis

Q9UUS2

-

Gaeumannomyces graminis

mycelium

-

Gaeumannomyces graminis

-

1.8

-

pH 7.4, 0°C

Gaeumannomyces graminis

-80°C, stable

Gaeumannomyces graminis

linoleate + O2

-

285243

Gaeumannomyces graminis

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

linoleate + O2

H2O2 (up to 9 mM) does not support enzyme activity under anaerobic conditions

285243

Gaeumannomyces graminis

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

tetramer

4 * 130000, gel filtration

Gaeumannomyces graminis

15

-

linoleate

pH 7.4, 0°C

Gaeumannomyces graminis

7.2

7.4

-

Gaeumannomyces graminis

heme

the enzyme contains at least 2 mol of heme/mol of enzyme

Gaeumannomyces graminis

chromatofocusing

-

5.2

heme

the enzyme contains at least 2 mol of heme/mol of enzyme

Gaeumannomyces graminis

repeated freezing and thawing, particularly of dilute enzyme solutions, inactivates the enzyme

Gaeumannomyces graminis

the enzyme is quite unstable at the early steps of purification, but active fractions from anion-exchange chromatography can be kept for 1 week on ice with only a small loss of enzyme activity

Gaeumannomyces graminis

additional information

not inhibited by 1 mM KCN. CO does not inhibit the enzyme

Gaeumannomyces graminis

N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid

i.e. BW A4C, more than 90% inhibition at 0.01 mM, about 50% inhibition at 0.001 mM

Gaeumannomyces graminis

0.008

-

linoleate

pH 7.4 25°C

Gaeumannomyces graminis

0.03

-

O2

pH 7.4 25°C

Gaeumannomyces graminis

130000

-

4 * 130000, gel filtration

Gaeumannomyces graminis

520000

-

gel filtration

Gaeumannomyces graminis

linoleate + O2

Gaeumannomyces graminis

-

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

urea

the enzyme can be incubated in 4 M urea for 1 h, followed by desalting, with only partial loss of enzyme activity

Gaeumannomyces graminis

-

Gaeumannomyces graminis

mycelium

-

Gaeumannomyces graminis

-

1.8

-

pH 7.4, 0°C

Gaeumannomyces graminis

-80°C, stable

Gaeumannomyces graminis

linoleate + O2

-

285243

Gaeumannomyces graminis

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

linoleate + O2

H2O2 (up to 9 mM) does not support enzyme activity under anaerobic conditions

285243

Gaeumannomyces graminis

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate

-

?

tetramer

4 * 130000, gel filtration

Gaeumannomyces graminis

15

-

linoleate

pH 7.4, 0°C

Gaeumannomyces graminis

7.2

7.4

-

Gaeumannomyces graminis

chromatofocusing

-

5.2

741712

Seo

Characterization of a recombi ...

Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961

Appl. Microbiol. Biotechnol.

100

3087-3099

2016

1

-

1

-

11

-

17

-

1

5

-

1

-

1

8

-

25

3

1

2

-

1

18

3

-

1

-

1

-

11

-

17

-

1

-

1

-

1

8

-

25

3

2

-

1

18

3

-

20

742730

Seo

Production of 7,8-dihydroxy u ...

Colletotrichum gloeosporioides, Colletotrichum gloeosporioides KACC 40961

J. Agric. Food Chem.

64

8555-8562

2016

1

-

1

-

2

-

6

-

1

-

1

-

14

-

3

1

-

1

-

1

-

1

-

2

-

1

-

1

-

14

-

1

-

1

-

743200

Sooman

Discovery of a novel linoleat ...

Colletotrichum graminicola, Colletotrichum graminicola M1.001, Colletotrichum graminicola M1.001 / M2 / FGSC 10212, Fusarium oxysporum, Fusarium oxysporum Fo5176

Lipids

50

1243-1252

2015

-

1

-

5

-

13

-

29

-

8

2

-

1

-

5

-

29

-

2

-

2

-

724132

Hoffmann

7,8- and 5,8-linoleate diol sy ...

Aspergillus fumigatus, Gaeumannomyces graminis

Arch. Biochem. Biophys.

539

87-91

2013

-

2

-

7

-

4

-

5

2

4

-

2

-

7

-

5

2

-

2

-

711024

Hoffmann

Expression of 5,8-LDS of Asper ...

Aspergillus fumigatus, Gaeumannomyces graminis

Arch. Biochem. Biophys.

506

216-222

2010

-

2

-

6

-

2

-

3

-

4

-

2

-

10

-

10

-

2

-

7

-

2

-

3

-

2

-

10

-

2

-

714317

Jernerén

Reaction mechanism of 5,8-lino ...

Aspergillus clavatus

Biochim. Biophys. Acta

1801

503-507

2010

-

1

-

4

-

2

-

3

-

1

-

2

-

704466

Brodhun

Identification of PpoA from As ...

Aspergillus nidulans

J. Biol. Chem.

284

11792-11805

2009

-

1

-

2

-

1

4

-

2

1

-

1

-

1

-

1

-

7

1

-

4

1

-

2

-

1

2

-

2

-

1

-

4

-

2

1

-

1

-

1

-

7

1

-

4

1

-

4

704475

Garscha

Leucine/valine residues direct ...

Gaeumannomyces graminis

J. Biol. Chem.

284

13755-13765

2009

-

1

-

4

-

1

-

2

-

2

-

2

-

1

-

4

-

1

-

2

-

702018

Garscha

Pichia expression and mutagene ...

Gaeumannomyces graminis, Gaeumannomyces graminis var. avenae

Biochem. Biophys. Res. Commun.

373

579-583

2008

-

1

-

3

-

2

-

4

-

1

-

4

-

2

-

1

-

3

-

2

-

1

-

4

-

703700

Garscha

Critical amino acids for the 8 ...

Gaeumannomyces graminis

FEBS Lett.

582

3547-3551

2008

-

1

-

11

-

1

-

1

-

3

-

1

-

1

-

11

-

1

-

285246

Hörnsten

Cloning of linoleate diol synt ...

Gaeumannomyces graminis

J. Biol. Chem.

274

28219-28224

1999

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

285251

Oliw

Catalytic properties of linole ...

Gaeumannomyces graminis

Adv. Exp. Med. Biol.

469

679-685

1999

2

-

2

-

1

-

2

-

1

-

1

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

285244

Su

A protein radical and ferryl i ...

Gaeumannomyces graminis

J. Biol. Chem.

273

20744-20751

1998

-

1

-

2

-

4

-

1

-

1

-

5

-

1

-

2

-

2

-

2

-

2

-

2

-

5

-

285243

Su

Purification and characterizat ...

Gaeumannomyces graminis

J. Biol. Chem.

271

14112-14118

1996

-

2

-

2

1

-

1

-

1

2

1

-

1

-

1

-

1

-

1

-

2

-

2

-

2

-

2

1

-

1

-

1

2

1

-

1

-

1

-

285249

Su

Studies on linoleic acid 8R-di ...

Gaeumannomyces graminis

Lipids

30

43-50

1995

-

1

16

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

16

-

1

-

1

-

1

-

1

-

1

-

285247

Brodowsky

BW A4C and other hydroxamic ac ...

Gaeumannomyces graminis

Eur. J. Pharmacol.

254

43-47

1994

-

12

-

1

-

2

-

1

-

1

-

1

-

1

-

6

-

6

12

-

1

-

1

-

1

-

1

-

1

-

285248

Brodowsky

Linoleic acid 8R-dioxygenase a ...

Gaeumannomyces graminis

Ann. N. Y. Acad. Sci.

744

314-316

1994

-

5

-

1

-

2

-

1

-

2

-

5

-

5

-

1

-

1

-

285250

Hamberg

Sequential oxygenation of lino ...

Gaeumannomyces graminis

Arch. Biochem. Biophys.

309

77-80

1994

-

2

-

1

-

4

-

2

-

4

-

285245

Brodowsky

A linoleic acid (8R)-dioxygena ...

Gaeumannomyces graminis

J. Biol. Chem.

267

14738-14745

1992

-

1

-

1

-

1

-

1

-

1

-

Purification and characterization of linoleate 8-dioxygenase from the fungus Gaeumannomyces graminis as anovel hemoprotein

Data extracted from this reference: