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Literature summary for 1.13.11.6 extracted from

  • Muraki, T.; Taki, M.; Hasegawa, Y.; Iwaki, H.; Lau, P.C.
    Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7 (2003), Appl. Environ. Microbiol., 69, 1564-1572.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
H52A inactive enzyme Pseudomonas fluorescens
H93A 24.8% activity of the native enzyme Pseudomonas fluorescens
H96A inactive enzyme Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline Fe2+ chelator, 1 mM, complete inhibition Pseudomonas fluorescens
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide methiodide carboxyl-directed reagent, 1 mM, 26% inhibition Pseudomonas fluorescens
2,2'-dipyridyl Fe2+ chelator, 1 mM, complete inhibition Pseudomonas fluorescens
Cd2+ 0.1 mM, more than 99% inhibition Pseudomonas fluorescens
Co2+ 1 mM, 92% inhibition Pseudomonas fluorescens
Cu2+ 0.1 mM, complete inhibition Pseudomonas fluorescens
diethyl dicarbonate modifies histidine residues of catechol dioxygenases, 1 mM, 70% inhibition Pseudomonas fluorescens
Dithionitrobenzoic acid cysteine-directed reagent, 1 mM, complete inhibition Pseudomonas fluorescens
EDTA 1 mM, 99% inhibition Pseudomonas fluorescens
Fe3+ 0.1 mM, 82% inhibition Pseudomonas fluorescens
iodoacetate cysteine-directed reagent, 1 mM, 42% inhibition Pseudomonas fluorescens
Ni2+ 1 mM, 82% inhibition Pseudomonas fluorescens
Zn2+ 0.1 mM, 99% inhibition Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ needed for catalytic activity Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21240
-
predicted from cDNA sequence Pseudomonas fluorescens
23800
-
SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxyanthranilate + O2 Pseudomonas fluorescens
-
2-amino-3-carboxymuconate semialdehyde
-
?
3-hydroxyanthranilate + O2 Pseudomonas fluorescens KU-7
-
2-amino-3-carboxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q83V26
-
-
Pseudomonas fluorescens KU-7 Q83V26
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.9
-
3-hydroxyanthranilate as substrate, pH 6.5, 30ºC Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyanthranilate + O2
-
Pseudomonas fluorescens 2-amino-3-carboxymuconate semialdehyde
-
?
3-hydroxyanthranilate + O2
-
Pseudomonas fluorescens KU-7 2-amino-3-carboxymuconate semialdehyde
-
?
additional information no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates Pseudomonas fluorescens ?
-
?
additional information no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates Pseudomonas fluorescens KU-7 ?
-
?