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Literature summary for 1.13.11.6 extracted from

  • Savage, N.; Levy, P.R.
    The purification and some properties of 3-hydroxyanthranilate oxygenase from baboon liver (1975), Int. J. Biochem., 6, 459-466.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuriphenyl sulfonic acid
-
Papio ursinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
4-Propyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.011
-
4-ethyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.037
-
4-methyl-3-hydroxyanthranilic acid
-
Papio ursinus
0.105
-
3-hydroxyanthranilic acid
-
Papio ursinus
0.615
-
O2
-
Papio ursinus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ requirement Papio ursinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000 40000 gel filtration, SDS-PAGE Papio ursinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxyanthranilate + O2 Papio ursinus enzyme occurs in the metabolic pathway of the conversion of tryptophan to nicotinic acid 2-amino-3-carboxymuconate semialdehyde
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information enzyme is not stable, when stored frozen in 20 mM Tris-maleate buffer, 1 mM dithiothreitol and 1 mM FeSO4 at pH 6.5 Papio ursinus

Organism

Organism UniProt Comment Textmining
Papio ursinus
-
baboon
-

Purification (Commentary)

Purification (Comment) Organism
-
Papio ursinus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Papio ursinus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
7.8
-
-
Papio ursinus

Storage Stability

Storage Stability Organism
4┬░C, overnight, 75% loss of activity Papio ursinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyanthranilate + O2
-
Papio ursinus 2-amino-3-carboxymuconate semialdehyde
-
?
3-hydroxyanthranilate + O2 enzyme occurs in the metabolic pathway of the conversion of tryptophan to nicotinic acid Papio ursinus 2-amino-3-carboxymuconate semialdehyde
-
?
4-ethyl-3-hydroxyanthranilate + O2
-
Papio ursinus 2-amino-3-carboxy-4-ethylmuconate semialdehyde
-
?
4-methyl-3-hydroxyanthranilate + O2
-
Papio ursinus 2-amino-3-carboxy-4-methylmuconate semialdehyde
-
?
4-propyl-3-hydroxyanthranilic acid + O2
-
Papio ursinus 2-amino-3-carboxy-4-propylmuconate semialdehyde
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000-40000, SDS-PAGE Papio ursinus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Papio ursinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6
-
Papio ursinus