Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.6 extracted from

  • Decker, R.H.; Kang, H.H.; Leach, F.R.; Henderson, L.M.
    Purification and properties of 3-hydroxyanthranilic acid oxidase (1961), J. Biol. Chem., 236, 3076-3082.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100
-
Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
3-hydroxyanthranilic acid
-
Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 the most effective salt, the optimal concentration is 0.035 M Bos taurus
Fe2+ solvent, acid and heat function to modify the protein configuration so that ferrous ions can be bound to the enzyme to generate the most active form Bos taurus
HCl during purification of enzyme treatment with acid was used Bos taurus
MgCl2
-
Bos taurus
NaCl
-
Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information drastic change in the Km of the enzyme in the presence of dimethylglutarate buffer Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
reaches a maximum after 10 min at acid pH Bos taurus

Storage Stability

Storage Stability Organism
-15┬░C, in 0.01 M Tris buffer, pH 7.0, 4 days, no loss of activity Bos taurus
4┬░C, overnight, about 75% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyanthranilate + O2
-
Bos taurus 2-amino-3-carboxymuconate semialdehyde
-
?

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
55
-
activation by heating at 55┬░C for 5 min Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
at pH 3.4: the enzyme is in a form which can bind substrate but is enzymatically inactive, at pH 6.5: active form of enzyme Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
4┬░C, half-life: 3 days Bos taurus