Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sphingobium sp. SYK-6 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Methoxy-4-methylphenol | 20.8% inhibition at 5 mM | Sphingobium sp. SYK-6 | |
4-nitrocatechol | 31.6% inhibition at 5 mM | Sphingobium sp. SYK-6 | |
protocatechuate | most potent inhibitor with mixed (competitive and non-competitive) inhibition profile, 49% inhibition at 5 mM | Sphingobium sp. SYK-6 | |
syringaldehyde | least potent inhibitor, 8.66% inhibition at 5 mM | Sphingobium sp. SYK-6 | |
syringate | 16.3% inhibition at 5 mM | Sphingobium sp. SYK-6 | |
vanilliate | 24.2% inhibition at 5 mM | Sphingobium sp. SYK-6 | |
Vanillin | 11.3% inhibition at 5 mM | Sphingobium sp. SYK-6 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.123 | - |
gallate | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. SYK-6 | G2IKE5 | - |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-25 gel filtration | Sphingobium sp. SYK-6 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gallate + O2 | - |
Sphingobium sp. SYK-6 | (2E,4E)-4-(carboxymethylidene)-2-hydroxypent-2-enedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49220, calculated from amino acid sequence | Sphingobium sp. SYK-6 |
Synonyms | Comment | Organism |
---|---|---|
DesB | - |
Sphingobium sp. SYK-6 |
gallate 3,4-dioxygenase | - |
Sphingobium sp. SYK-6 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
316 | - |
gallate | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.48 | - |
protocatechuate | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 | |
8.13 | - |
vanilliate | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 | |
13.3 | - |
4-nitrocatechol | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 |