Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sphingobium sp. SYK-6 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrocatechol | 36.6% inhibition when the inhibitor is present in 5fold excess over substrate | Sphingobium sp. SYK-6 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
gallate | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Sphingobium sp. SYK-6 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. SYK-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose column chromatography | Sphingobium sp. SYK-6 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gallate + O2 | - |
Sphingobium sp. SYK-6 | (2E,4E)-4-(carboxymethylidene)-2-hydroxypent-2-enedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49220, calculated from amino acid sequence | Sphingobium sp. SYK-6 |
Synonyms | Comment | Organism |
---|---|---|
DesB | - |
Sphingobium sp. SYK-6 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.8 | - |
gallate | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
4-nitrocatechol | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
398 | - |
gallate | at pH 7.5, temperature not specified in the publication | Sphingobium sp. SYK-6 |