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Literature summary for 1.13.11.57 extracted from

  • Uchendu, S.N.; Rafalowski, A.; Cohn, E.F.; Davoren, L.W.; Taylor, E.A.
    Anaerobic protein purification and kinetic analysis via oxygen electrode for studying DesB dioxygenase activity and inhibition (2018), J. Vis. Exp., 140, 58307 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sphingobium sp. SYK-6

Inhibitors

Inhibitors Comment Organism Structure
4-nitrocatechol 36.6% inhibition when the inhibitor is present in 5fold excess over substrate Sphingobium sp. SYK-6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
gallate at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Sphingobium sp. SYK-6

Organism

Organism UniProt Comment Textmining
Sphingobium sp. SYK-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose column chromatography Sphingobium sp. SYK-6

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gallate + O2
-
Sphingobium sp. SYK-6 (2E,4E)-4-(carboxymethylidene)-2-hydroxypent-2-enedioate
-
?

Subunits

Subunits Comment Organism
? x * 49220, calculated from amino acid sequence Sphingobium sp. SYK-6

Synonyms

Synonyms Comment Organism
DesB
-
Sphingobium sp. SYK-6

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.8
-
gallate at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3
-
4-nitrocatechol at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
398
-
gallate at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6