Literature summary for 1.13.11.55 extracted from

Sato, Y.; Yabuki, T.; Adachi, N.; Moriya, T.; Arakawa, T.; Kawasaki, M.; Yamada, C.; Senda, T.; Fushinobu, S.; Wakagi, T.
Crystallographic and cryogenic electron microscopic structures and enzymatic characterization of sulfur oxygenase reductase from Sulfurisphaera tokodaii (2020), J. Struct. Biol., X 4, 100030 .

Search for more literature for 1.13.11.55

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure at 1.73 A resolution. At the catalytic center, iron is ligated to His86, His90, Glu114, and two water molecules. Three conserved cysteines in the cavity are located 9.5-13 A from the iron and are observed as free thiol forms. The iron and Cys31 are essential	Sulfurisphaera tokodaii

Protein Variants

Protein Variants	Comment	Organism
C101A	about 10% residual activtiy for both oxygenase and reductase activity	Sulfurisphaera tokodaii
C104A	about 10% residual activtiy for both oxygenase and reductase activity	Sulfurisphaera tokodaii
C31A	complete loss of activity	Sulfurisphaera tokodaii
E114A	complete loss of activity	Sulfurisphaera tokodaii
H86A	complete loss of activity	Sulfurisphaera tokodaii
H90A	complete loss of activity	Sulfurisphaera tokodaii

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Sulfurisphaera tokodaii
cyanide	1 mM, 71% inhibition	Sulfurisphaera tokodaii
dithiothreitol	inhibits both reactions	Sulfurisphaera tokodaii
Fe2+	inhibits oxygenase reaction	Sulfurisphaera tokodaii
Fe3+	inhibits oxygenase reaction	Sulfurisphaera tokodaii
Hg2+	-	Sulfurisphaera tokodaii
iodoacetic acid	inhibits both reactions	Sulfurisphaera tokodaii
additional information	not inhibitory: sodium azide	Sulfurisphaera tokodaii
N-ethylmaleimide	inhibits both reactions	Sulfurisphaera tokodaii
PCMB	inhibits both reactions	Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	at the catalytic center, iron is ligated to His86, His90, Glu114, and two water molecules	Sulfurisphaera tokodaii
Zn2+	activating	Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
539000	-	gel filtration	Sulfurisphaera tokodaii

Organism

Organism	UniProt	Comment	Textmining
Sulfurisphaera tokodaii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, by heat treatment and chromatography steps with four different columns	Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.95	-	80°C, pH 6, production of hydrogen sulfite	Sulfurisphaera tokodaii
10.5	-	80°C, pH 6, oxygenase reaction	Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3S + 3H2O	-	Sulfurisphaera tokodaii	HSO3- + 2HS- + 3H+	-	?
4 sulfur + 4 H2O + O2	-	Sulfurisphaera tokodaii	2 hydrogen sulfide + 2 sulfite	-	?

Subunits

Subunits	Comment	Organism
multimer	15-16 * 38000, SDS-PAGE, 15-16 * 35700, calculated from sequence	Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	30 min, the remaining oxygenase and reductase activities are approximately 40% and 90%, respectively	Sulfurisphaera tokodaii

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Release 2023.1 (January 2023)