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Literature summary for 1.13.11.55 extracted from

  • Urich, T.; Bandeiras, T.M.; Leal, S.S.; Rachel, R.; Albrecht, T.; Zimmermann, P.; Scholz, C.; Teixeira, M.; Gomes, C.M.; Kletzin, A.
    The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre (2004), Biochem. J., 381, 137-146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
EDTA 100 mM, slight stimulatory effect Acidianus ambivalens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli results in active, soluble SOR and in inclusion bodies from which active SOR can be refolded as long as ferric ions are present in the refolding solution. Wild-type, recombinant and refolded enzyme possesses indistinguishable properties Acidianus ambivalens

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 0.5 mM, 95% inhibition of oxygenase reaction (formation of hydrogen sulfide), 84% inhibition of reductase reaction (formation of sulfite plus thiosulfate) Acidianus ambivalens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 sulfur pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide) Acidianus ambivalens
13
-
sulfur pH 7.2, 65°C, reductase reaction (formation of sulphite plus thiosulfate) Acidianus ambivalens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains a low-potential mononuclear non-heme iron centre, which has a reduction potential of -268 mV at pH 6.5, one iron atom per subunit Acidianus ambivalens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
24 * 36000, ball-shaped assembly with a central hollow core probably consisting of 24 subunits in a 432 symmetry, the subunits form homodimers as the building blocks of the holoenzyme, SDS-PAGE Acidianus ambivalens
732000
-
non-denaturing PAGE Acidianus ambivalens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 sulfur + 4 H2O + O2 Acidianus ambivalens initial enzyme in the sulfur-oxidation pathway 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?
4 sulfur + 4 H2O + O2 Acidianus ambivalens DSM 3772 initial enzyme in the sulfur-oxidation pathway 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl reversible denaturation curve with midpoint at 3.4 M guanidine-HCl Acidianus ambivalens
urea reversible denaturation curve with midpoints at 5.9 M urea Acidianus ambivalens

Organism

Organism UniProt Comment Textmining
Acidianus ambivalens P29082
-
-
Acidianus ambivalens DSM 3772 P29082
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acidianus ambivalens

Renatured (Commentary)

Renatured (Comment) Organism
reversible denaturation curves with midpoints at 3.4 M guanidine-HCl and 5.9 M urea Acidianus ambivalens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 sulfur + 4 H2O + O2
-
Acidianus ambivalens 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?
4 sulfur + 4 H2O + O2 initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?
4 sulfur + 4 H2O + O2
-
Acidianus ambivalens DSM 3772 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?
4 sulfur + 4 H2O + O2 initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens DSM 3772 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?
additional information not active with tetrathionate Acidianus ambivalens ?
-
?
additional information not active with tetrathionate Acidianus ambivalens DSM 3772 ?
-
?

Subunits

Subunits Comment Organism
tetraicosamer 24 * 36000, ball-shaped assembly with a central hollow core probably consisting of 24 subunits in a 432 symmetry, the subunits form homodimers as the building blocks of the holoenzyme, SDS-PAGE Acidianus ambivalens

Synonyms

Synonyms Comment Organism
SOR
-
Acidianus ambivalens
sulphur oxygenase reductase
-
Acidianus ambivalens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
sulfur pH 7.2, 65°C, reductase reaction (formation of sulfite plus thiosulfate) Acidianus ambivalens
2.2
-
sulfur pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide) Acidianus ambivalens

General Information

General Information Comment Organism
physiological function initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens