Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) codon Plus RIPL cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with selenomethionine, sitting drop vapor diffusion method, using 1:0.8 sodium acetate (0.1 M), ammonium sulfate (2.0 M pH 4.5) | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 | Homo sapiens | - |
4-(methylsulfanyl)-2-oxobutanoate + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BV57 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 | - |
Homo sapiens | 4-(methylsulfanyl)-2-oxobutanoate + formate | - |
? | |
1,2-dihydroxy-hex-1-en-3-one + O2 | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fe-ARD | - |
Homo sapiens |