Crystallization (Comment) | Organism |
---|---|
Ni- and Co-bound proteins. Both Ni and Co present an octahedral coordination geometry in the active site bound to protein ligands H88, H90, H133 and E94. Additionally, two distinct water (or hydroxide) ligands bind to the metal ion center | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1185 | - |
(1Z)-1,2-dihydroxyhex-1-en-3-one | Fe2+ bound enzyme, pH 7.0, 25°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the Ni2+ bound protein catalyzes the reaction of EC 1.13.11.53 | Mus musculus | |
Fe2+ | Ni2+ bound ARD is the most stable followed by Co2+ and Fe2+, and Mn2+-bound ARD being the least stable | Mus musculus | |
Mn2+ | the Ni2+ bound protein catalyzes the reaction of EC 1.13.11.53 | Mus musculus | |
additional information | the identity of bound metal ion does not affect the oligomeric state of ARD | Mus musculus | |
Ni2+ | the Ni2+ bound protein catalyzes the reaction of EC 1.13.11.53 | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | 26000 | gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q99JT9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1Z)-1,2-dihydroxyhex-1-en-3-one + O2 | i.e. desthio-acireductone | Mus musculus | 2-oxovalerate + formic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21500, calculated | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
Mn2+ bound enzyme, melting temperature | Mus musculus |
58 | - |
Ni2+ bound enzyme, melting temperature | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
111.7 | - |
(1Z)-1,2-dihydroxyhex-1-en-3-one | Fe2+ bound enzyme, pH 7.0, 25°C | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
950 | - |
(1Z)-1,2-dihydroxyhex-1-en-3-one | Fe2+ bound enzyme, pH 7.0, 25°C | Mus musculus |