Literature summary for 1.13.11.52 extracted from

Pearson, J.T.; Siu, S.; Meininger, D.P.; Wienkers, L.C.; Rock, D.A.
In vitro modulation of cytochrome P450 reductase supported indoleamine 2,3-dioxygenase activity by allosteric effectors cytochrome b5 and methylene blue (2010), Biochemistry, 49, 2647-2656.

Search for more literature for 1.13.11.52

Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbic acid	-	Homo sapiens
cytochrome b5	-	Homo sapiens
methylene blue	-	Homo sapiens
NADPH-cytochrome P450 reductase	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1-Methyltryptophan	-	Homo sapiens
annulin B	-	Homo sapiens
menadione	menadione effectively inhibits L-Trp oxidation from reactions supported with ascorbaic acid/methylene blue	Homo sapiens
methylene blue	addition of methylene blue (0-0.1 mM) to NADPH-cytochrome P450 reductase-supported D-Trp incubations (with or without cytochrome b5) leads to concentration-dependent inhibition of IDO activity, addition of methylene blue (0-0.03 mM) to NADPH-cytochrome P450 reductase-supported L-Trp oxidation reactions results in a switch from substrate inhibition kinetics to Michaelis-Menten with increasing methylene blue concentration decreasing the affinity of IDO for L-Trp	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00072	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0015	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and in the presence of 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0017	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0019	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0039	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.004	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0049	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0054	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0124	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0129	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0224	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
0.0279	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-tryptophan + O2	Homo sapiens	-	N-formyl-D-kynurenine	-	?
L-tryptophan + O2	Homo sapiens	-	N-formyl-L-kynurenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P14902	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-tryptophan + O2	-	Homo sapiens	N-formyl-D-kynurenine	-	?
L-tryptophan + O2	-	Homo sapiens	N-formyl-L-kynurenine	-	?

Synonyms

Synonyms	Comment	Organism
IDO	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.87	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
1.3	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
1.7	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and in the presence of 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
2.2	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
2.5	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
3	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
3.4	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
4.2	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
6.4	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
8.1	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
10	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
12	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00064	-	in PBS buffer (pH 7.4), at 37°C	Homo sapiens	menadione

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
290	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
490	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
540	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
610	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
640	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
740	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
760	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
800	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
850	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
1000	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
1100	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and in the presence of 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens
1200	-	L-tryptophan	NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and cytochrome b5, in PBS buffer (pH 7.4), at 37°C	Homo sapiens

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Release 2023.1 (January 2023)