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Literature summary for 1.13.11.52 extracted from

  • Forouhar, F.; Anderson, J.L.; Mowat, C.G.; Vorobiev, S.M.; Hussain, A.; Abashidze, M.; Bruckmann, C.; Thackray, S.J.; Seetharaman, J.; Tucker, T.; Xiao, R.; Ma, L.C.; Zhao, L.; Acton, T.B.; Montelione, G.T.; Chapman, S.K.; Tong, L.
    Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase (2007), Proc. Natl. Acad. Sci. USA, 104, 473-478.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.52

Cloned(Commentary)

Cloned (Comment) Organism
full-length enzyme is cloned into a pET-21d derivative, with a C-terminal hexahistidine tag, and overexpressed at 17°C in Escherichia coli BL21(DE3) pMGK cells Xanthomonas campestris

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor diffusion method, crystal structures at up to 1.6-A resolution of the active, reduced (Fe(II)) enzyme in a binary complex with the substrate L-Trp or 6-fluoro-Trp Xanthomonas campestris

Protein Variants

Protein Variants Comment Organism
H55A kcat for L-Trp is 6.8fold lower than wild-type enzyme, KM-value is 1.7fold higher than wild-type value Xanthomonas campestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.114
-
 L-Trp 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
100
-
 5-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
119
-
 O2 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
133
-
 L-Trp 25°C, pH 7.5, mutant enzyme H55A Xanthomonas campestris
186
-
 6-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris Q8PDA8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluoro-DL-tryptophan + O2
-
 Xanthomonas campestris ?
-
 ?
6-fluoro-DL-tryptophan + O2
-
 Xanthomonas campestris ?
-
 ?
L-Trp + O2
-
 Xanthomonas campestris N-formyl-L-kynurenine
-
 ?
additional information no activity with D-Trp and indolepropionic acid Xanthomonas campestris ?
-
 ?

Synonyms

Synonyms Comment Organism
TDO
-
 Xanthomonas campestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
 5-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
2.86
-
 L-Trp 25°C, pH 7.5, mutant enzyme H55A Xanthomonas campestris
3 6 L-Trp 25°C, pH 7.5, mutant enzyme H55A Xanthomonas campestris
19.5
-
 L-Trp 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
35.4
-
 O2 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
37.3
-
 6-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris

Cofactor

Cofactor Comment Organism Structure
heme
-
 Xanthomonas campestris