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Literature summary for 1.13.11.52 extracted from

  • Watanabe, Y.; Fujiwara, M.; Yoshida, R.; Hayaishi, O.
    Stereospecificity of hepatic L-tryptophan 2,3-dioxygenase (1980), Biochem. J., 189, 393-405.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.52

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxy-D-tryptophan 0.1 mM, 26% inhibition of cleavage of D-tryptophan, 29% inhibition of cleavage of L-tryptophan Mus musculus
5-hydroxy-L-tryptophan 0.1 mM, 40% inhibition of cleavage of D-tryptophan, 53% inhibition of cleavage of L-tryptophan Mus musculus
indole-3-acetic acid 1 mM, 19% inhibition of cleavage of D-tryptophan, 27% inhibition of cleavage of L-tryptophan Mus musculus
indole-3-acrylic acid competitive inhibitor Mus musculus
iodoacetic acid 1 mM, 12% inhibition of cleavage of D-tryptophan, 7% inhibition of cleavage of L-tryptophan Mus musculus
KCN 0.01 mM, 39% inhibition of cleavage of D-tryptophan, 48% inhibition of cleavage of L-tryptophan Mus musculus
additional information not inhibited by superoxide dismutase Mus musculus
N-ethylmaleimide 1 mM, 58% inhibition of cleavage of D-tryptophan, 45% inhibition of cleavage of L-tryptophan Mus musculus
NaN3 10 mM, 33% inhibition of cleavage of D-tryptophan, 32% inhibition of cleavage of L-tryptophan Mus musculus
serotonin 1 mM, 36% inhibition of cleavage of D-tryptophan, 37% inhibition of cleavage of L-tryptophan Mus musculus
Tiron 10 mM, 2% inhibition of cleavage of D-tryptophan, 6% inhibition of cleavage of L-tryptophan Mus musculus
tryptamine 0.1 mM, 16% inhibition of cleavage of D-tryptophan, 11% inhibition of cleavage of L-tryptophan Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 L-tryptophan
-
 Mus musculus
0.96
-
 D-tryptophan
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 alpha2beta2 subunit Mus musculus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Capra hircus
-
-
-
Homo sapiens
-
-
-
Macaca fuscata fuscata
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Pseudomonas fluorescens
-
-
-
Rattus norvegicus
-
 Sprague Dawley
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydroxyapatite column chromatography (two times), gel filtration, DEAE cellulose column chromatography, third hydroxyapatite column chromatography, Sephadex G-200 gel filtration, second DEAE cellulose column chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-
liver
-
 Bos taurus
-
liver
-
 Oryctolagus cuniculus
-
liver
-
 Capra hircus
-
liver
-
 Macaca fuscata fuscata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
 25°C, 50 mM HEPES/NaOH (pH 7.3), 5 mM D-tryptophan 0.01 mM methylene blue, 5 mM ascorbic acid, 0.001 mM catalase Mus musculus
2.13
-
 25°C, 50 mM HEPES/NaOH (pH 7.3), 2 mM L-tryptophan 0.01 mM methylene blue, 5 mM ascorbic acid, 0.001 mM catalase Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-tryptophan + O2
-
 Mus musculus N-formyl-D-kynurenine
-
 ?
L-tryptophan + O2
-
 Mus musculus N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Homo sapiens N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Rattus norvegicus N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Sus scrofa N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Bos taurus N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Oryctolagus cuniculus N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Pseudomonas fluorescens N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Capra hircus N-formyl-L-kynurenine
-
 ?
L-tryptophan + O2
-
 Macaca fuscata fuscata N-formyl-L-kynurenine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 25°C Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0045
-
 indole-3-acrylic acid against L-tryptophan Mus musculus
0.0068
-
 indole-3-acrylic acid against D-tryptophan Mus musculus