Literature summary for 1.13.11.52 extracted from

Austin, C.J.; Mizdrak, J.; Matin, A.; Sirijovski, N.; Kosim-Satyaputra, P.; Willows, R.D.; Roberts, T.H.; Truscott, R.J.; Polekhina, G.; Parker, M.W.; Jamie, J.F.
Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase (2004), Protein Expr. Purif., 37, 392-398.

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Application

Application	Comment	Organism
medicine	first and rate-limiting enzyme in tryptophan metabolism, plays a role in pathogenesis of many diseases	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli E538, formation of inclusion bodies at 37°C with reduced formation, at 30°C, expressed with His-Tag	Homo sapiens
expression of His-tagged enzyme in Escherichia coli, optimised expression	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	first and rate-limiting enzyme in tryptophan metabolism	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA-agarose and gel filtration (Superdex 75)	Homo sapiens
the use of ALA, the biosynthetic precursor of protoporphyrin IX, coupled with metal-affinity chromatography and size exclusion chromatography produces 6His-IDP with a protein to heme ratio of 1:2.2	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.67	-	-	Homo sapiens
2.67	-	kynurenine, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.2 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 60 min	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	first and rate-limiting enzyme in tryptophan metabolism	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
IDO	-	Homo sapiens
indoleamine 2,3-dioxygenase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	hemoprotein. The use of ALA, the biosynthetic precursor of protoporphyrin IX, coupled with metal-affinity chromatography and size exclusion chromatography produces 6His-IDP with a protein to heme ratio of 1:2.2	Homo sapiens

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Release 2023.1 (January 2023)