Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.50 extracted from

  • Straganz, G.D.; Glieder, A.; Brecker, L.; Ribbons, D.W.; Steiner, W.
    Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme from Acinetobacter johnsonii (2003), Biochem. J., 369, 573-581.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.50

Cloned(Commentary)

Cloned (Comment) Organism
-
 Acinetobacter johnsonii

Inhibitors

Inhibitors Comment Organism Structure
EDTA largely irreversible losses Acinetobacter johnsonii
H2O2 0.1 mM, immediate and total inhibition Acinetobacter johnsonii
hexacyanoferrate(III) 2.5 mM, 80% decrease in activity within 10 min Acinetobacter johnsonii
KCN largely irreversible losses Acinetobacter johnsonii
o-phenanthroline largely irreversible losses Acinetobacter johnsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000009
-
 Pentane-2,4-dione
-
 Acinetobacter johnsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.9-1.0 iron atoms per subunit, bound to active enzyme, essential for catalytic activity Acinetobacter johnsonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pentane-2,4-dione + O2 Acinetobacter johnsonii
-
 acetate + 2-oxopropanal
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter johnsonii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter johnsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dioxopentanoic acid ethyl ester + O2
-
 Acinetobacter johnsonii ?
-
 ?
2,4-octanedione + O2
-
 Acinetobacter johnsonii ?
-
 ?
2-acetylcyclohexanone + O2
-
 Acinetobacter johnsonii ?
-
 ?
3,5-heptanedione + O2
-
 Acinetobacter johnsonii ?
-
 ?
3-methyl-2,4-pentanedione + O2
-
 Acinetobacter johnsonii ?
-
 ?
3-oxobutanone + O2
-
 Acinetobacter johnsonii ?
-
 ?
4-hydroxy-4-methyl-2-pentanone + O2
-
 Acinetobacter johnsonii ?
-
 ?
ethylacetoacetate + O2
-
 Acinetobacter johnsonii ?
-
 ?
additional information no substrate: 4-hydroxy-4-methyl-2-pentanone, 3-oxobutanoate, quercetin, catechols, 2,4`-dihydroxyacetophenone, ascorbate Acinetobacter johnsonii ?
-
 ?
pentane-2,4-dione + O2
-
 Acinetobacter johnsonii acetate + 2-oxopropanal
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 x 16000, SDS-PAGE, MALDI-TOF MS Acinetobacter johnsonii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5
-
 Acinetobacter johnsonii