Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.5 extracted from

  • Bernini, A.; Galderisi, S.; Spiga, O.; Bernardini, G.; Niccolai, N.; Manetti, F.; Santucci, A.
    Toward a generalized computational workflow for exploiting transient pockets as new targets for small molecule stabilizers Application to the homogentisate 1,2-dioxygenase mutants at the base of rare disease alkaptonuria (2017), Comput. Biol. Chem., 70, 133-141 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A122D the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens
E42A the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens
H80Q the mutant shows slightly reduced specific activity compared to the wild type enzyme Homo sapiens
I216T the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens
M368V the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens
R225H the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens
S189I the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens
W60G the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens
Y62C the mutant shows strongly reduced specific activity compared to the wild type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains Fe2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
homogentisate + O2 Homo sapiens
-
4-maleylacetoacetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q93099
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0014
-
mutant enzyme I216T, pH and temperature not specified in the publication Homo sapiens
0.0132
-
mutant enzyme W60G, pH and temperature not specified in the publication Homo sapiens
0.023
-
mutant enzyme R225H, pH and temperature not specified in the publication Homo sapiens
0.7
-
mutant enzyme S189I, pH and temperature not specified in the publication Homo sapiens
4.708
-
mutant enzyme Y62C, pH and temperature not specified in the publication Homo sapiens
5.972
-
mutant enzyme E42A, pH and temperature not specified in the publication Homo sapiens
6.842
-
mutant enzyme A122D, pH and temperature not specified in the publication Homo sapiens
7.715
-
mutant enzyme M368V, pH and temperature not specified in the publication Homo sapiens
19.275
-
mutant enzyme H80Q, pH and temperature not specified in the publication Homo sapiens
20.932
-
wild type enzyme, pH and temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homogentisate + O2
-
Homo sapiens 4-maleylacetoacetate
-
?

Subunits

Subunits Comment Organism
homohexamer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
HgD
-
Homo sapiens