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Literature summary for 1.13.11.49 extracted from

  • Geeraerts, Z.; Celis, A.I.; Mayfield, J.A.; Lorenz, M.; Rodgers, K.R.; DuBois, J.L.; Lukat-Rodgers, G.S.
    Distinguishing active site characteristics of chlorite dismutases with their cyanide complexes (2018), Biochemistry, 57, 1501-1516 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner(DE3) cells Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
cyanide strong, competitive inhibitor Dechloromonas aromatica
cyanide strong, competitive inhibitor Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chloride + O2 Klebsiella pneumoniae
-
chlorite
-
?
chloride + O2 Dechloromonas aromatica
-
chlorite
-
?
chloride + O2 Klebsiella pneumoniae MGH 78578
-
chlorite
-
?

Organism

Organism UniProt Comment Textmining
Dechloromonas aromatica
-
-
-
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae MGH 78578
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chloride + O2
-
Klebsiella pneumoniae chlorite
-
?
chloride + O2
-
Dechloromonas aromatica chlorite
-
?
chloride + O2
-
Klebsiella pneumoniae MGH 78578 chlorite
-
?

Subunits

Subunits Comment Organism
homodimer
-
Klebsiella pneumoniae
homopentamer
-
Dechloromonas aromatica

Synonyms

Synonyms Comment Organism
chlorite dismutase
-
Klebsiella pneumoniae
chlorite dismutase
-
Dechloromonas aromatica
CLD
-
Klebsiella pneumoniae
CLD
-
Dechloromonas aromatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2600
-
chloride at pH 6.0 and 25°C Klebsiella pneumoniae
23000
-
chloride at pH 6.0 and 25°C Dechloromonas aromatica

Cofactor

Cofactor Comment Organism Structure
heme
-
Klebsiella pneumoniae
heme
-
Dechloromonas aromatica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9000
-
chloride at pH 6.0 and 25°C Klebsiella pneumoniae
40000
-
chloride at pH 6.0 and 25°C Dechloromonas aromatica