Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant N-terminally His6-tagged HODs in Escherichia coli strain M15 | Paenarthrobacter nitroguajacolicus |
Protein Variants | Comment | Organism |
---|---|---|
C69S | an oxidatively stable mutant variant | Paenarthrobacter nitroguajacolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nitroguajacolicus | - |
- |
- |
Paenarthrobacter nitroguajacolicus Rü61a | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant N-terminally His6-tagged HODs from Escherichia coli strain M15 by nickel affinity and anion exchange chromatography | Paenarthrobacter nitroguajacolicus |
Subunits | Comment | Organism |
---|---|---|
More | at 25°C, recombinant His6-tagged HodC has a hydrodynamic radius of 2.3 nm and an unusually high degree of alpha-helical structure of about 60%, based on deconvolution of CD spectra. The percentage of beta-sheets and -turns is expected to be relatively low in view of its sequence similarity to proteins of the alpha/beta-hydrolase fold superfamily, transition two-state model, overview | Paenarthrobacter nitroguajacolicus |
Synonyms | Comment | Organism |
---|---|---|
1H-3-Hydroxy-4-oxoquinaldine 2,4-dioxygenase | - |
Paenarthrobacter nitroguajacolicus |
HOD | - |
Paenarthrobacter nitroguajacolicus |
More | HOD belongs to the alpha/beta-hydrolase-fold superfamily of enzymes | Paenarthrobacter nitroguajacolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant His6HodC exhibits three-state unfolding with an intermediate state I that exhibits at the transition temperature a volume larger than that of the native or denatured state. The intermediate state I is also associated with the highest isothermal expansion coefficient, alphaP, of the three states and exhibits a significantly lower percentage of R-helical structure than the native state. The stability difference between the native and intermediate state is rather small which makes I a potential candidate for reactions with various ligands, particularly those having a preference for the apparently preserved beta-type motifs | Paenarthrobacter nitroguajacolicus |