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Literature summary for 1.13.11.47 extracted from

  • Block, D.W.; Lingens, F.
    Microbial metabolism of quinoline and related compounds. XIV. Purification and properties of 1H-3-hydroxy-4-oxoquinoline oxygenase, a new extradiol cleavage enzyme from Pseudomonas putida strain 33/1 (1992), Biol. Chem. Hoppe-Seyler, 373, 343-349.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Pseudomonas putida
2,2'-dipyridyl
-
 Pseudomonas putida
Cu2+
-
 Pseudomonas putida
Hg2+
-
 Pseudomonas putida
iodoacetate
-
 Pseudomonas putida
KCN
-
 Pseudomonas putida
KSCN
-
 Pseudomonas putida
PCMB
-
 Pseudomonas putida
Zn2+
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 1H-3-Hydroxy-4-oxoquinoline
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.9
-
-
 Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, stable for 3 days Pseudomonas putida
4°C, 15% loss of activity after 2 days Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1H-3-Hydroxy-4-oxoquinoline + O2
-
 Pseudomonas putida N-Formylanthranilate + CO
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida