BRENDA - Enzyme Database
show all sequences of 1.13.11.40

Inducible expression of 15-lipoxygenase-2 and 8-lipoxygenase inhibits cell growth via common signaling pathways

Schweiger, D.; Fuerstenberger, G.; Krieg, P.; J. Lipid Res. 48, 553-564 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression and establishment of a doxycycline-inducible Tet-On gene expression system in the premalignant mouse keratinocyte cell line 308
Mus musculus
Engineering
Protein Variants
Commentary
Organism
additional information
doxycycline-inducible expression in keratinocytes 308 leads to an inhibition of cell growth that is associated with an inhibition of DNA synthesis, as shown by a reduction of 5-bromo-2-deoxy-uridine incorporation up to 46%, doxycycline-induced keratinocytes show increased levels of reactive oxygen species. The antioxidant N-acetyl-L-cysteine and a specific inhibitor of p38 mitogen-activated protein kinase, but not of extracellular signal-regulated kinase 1/2 or c-Jun N-terminal kinase/stress-activated kinases, completely abolish the LOX-induced growth inhibition
Mus musculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
baicalein
-
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
arachidonate + O2
Mus musculus
-
(5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
-
-
?
additional information
Mus musculus
the inducible expression of 8-lipoxygenase inhibits cell growth, 15-LOX-2 and 8-LOX, although displaying different positional specificity, use common signaling pathways to induce growth inhibition in premalignant epithelial cells, overview
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mus musculus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
arachidonate + O2
-
688221
Mus musculus
(5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
-
-
-
?
additional information
the inducible expression of 8-lipoxygenase inhibits cell growth, 15-LOX-2 and 8-LOX, although displaying different positional specificity, use common signaling pathways to induce growth inhibition in premalignant epithelial cells, overview
688221
Mus musculus
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
8-lipoxygenase
-
Mus musculus
8-LOX
-
Mus musculus
More
the enzyme belongs to the LOX family
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression and establishment of a doxycycline-inducible Tet-On gene expression system in the premalignant mouse keratinocyte cell line 308
Mus musculus
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
doxycycline-inducible expression in keratinocytes 308 leads to an inhibition of cell growth that is associated with an inhibition of DNA synthesis, as shown by a reduction of 5-bromo-2-deoxy-uridine incorporation up to 46%, doxycycline-induced keratinocytes show increased levels of reactive oxygen species. The antioxidant N-acetyl-L-cysteine and a specific inhibitor of p38 mitogen-activated protein kinase, but not of extracellular signal-regulated kinase 1/2 or c-Jun N-terminal kinase/stress-activated kinases, completely abolish the LOX-induced growth inhibition
Mus musculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
baicalein
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
arachidonate + O2
Mus musculus
-
(5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
-
-
?
additional information
Mus musculus
the inducible expression of 8-lipoxygenase inhibits cell growth, 15-LOX-2 and 8-LOX, although displaying different positional specificity, use common signaling pathways to induce growth inhibition in premalignant epithelial cells, overview
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
arachidonate + O2
-
688221
Mus musculus
(5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
-
-
-
?
additional information
the inducible expression of 8-lipoxygenase inhibits cell growth, 15-LOX-2 and 8-LOX, although displaying different positional specificity, use common signaling pathways to induce growth inhibition in premalignant epithelial cells, overview
688221
Mus musculus
?
-
-
-
?
Other publictions for EC 1.13.11.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Biochemistry
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702435
Walther
Structural basis for pH-depend ...
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Biochim. Biophys. Acta
1791
827-835
2009
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688221
Schweiger
Inducible expression of 15-lip ...
Mus musculus
J. Lipid Res.
48
553-564
2007
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671715
Jisaka
Double dioxygenation by mouse ...
Mus musculus
Biochem. Biophys. Res. Commun.
338
136-143
2005
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674505
Oldham
Insights from the X-ray crysta ...
Plexaura homomalla
J. Biol. Chem.
280
39545-39552
2005
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659650
Schneider
Upregulation of 8-lipoxygenase ...
Mus musculus
J. Invest. Dermatol.
122
691-698
2004
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660365
Coffa
A single active site residue d ...
Plexaura homomalla
Proc. Natl. Acad. Sci. USA
101
15579-15584
2004
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439401
Qiao
Leukotriene A synthase activit ...
Mus musculus
Biochim. Biophys. Acta
1438
131-139
1999
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439400
Steel
Identification of an 8-lipoxyg ...
Aplysia californica
J. Biol. Chem.
272
18673-18681
1997
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439399
Brash
Purification and molecular clo ...
Asteroidea, Callista chione, Plexaura homomalla, Strongylocentrotus purpuratus
J. Biol. Chem.
271
20949-20957
1996
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439398
Fuerstenberger
Characterization of an 8-lipox ...
Mus musculus
J. Biol. Chem.
266
15738-15745
1991
2
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439397
Brash
On non-cyclooxygenase prostagl ...
Plexaura homomalla
J. Biol. Chem.
262
15829-15839
1987
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439396
Bundy
Discovery of an arachidonic ac ...
Pseudoplexaura porosa
J. Biol. Chem.
261
747-751
1986
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