Literature summary for 1.13.11.40 extracted from

Oldham, M.L.; Brash, A.R.; Newcomer, M.E.
Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality (2005), J. Biol. Chem., 280, 39545-39552.

Search for more literature for 1.13.11.40

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Plexaura homomalla

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion in 6.25% polyethylene glycol 8000, 100 mM imidazole acetate (pH 8.0), 100 mM CaCl2, and 5% sucrose at 22°C	Plexaura homomalla

Protein Variants

Protein Variants	Comment	Organism
D39A	118% activity compared to the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding	Plexaura homomalla
D39A/E47A	106% activity compared to the wild type enzyme, a double mutant with calcium-binding residues from two of the three sites mutated exhibits no fluorescence resonance energy transfer signal	Plexaura homomalla
E47A	65% of the activity of the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding	Plexaura homomalla
W41A	140% activity compared to the wild type enzyme, exhibits only less than 2% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal	Plexaura homomalla
W77A	44% of the activity of the wild type enzyme, exhibits only 4% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal	Plexaura homomalla

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	Ca2+-regulated enzyme activity	Plexaura homomalla

Organism

Organism	UniProt	Comment	Textmining
Plexaura homomalla	-	Caribbean sea whip coral	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-nitrilotriacetic acid-agarose chromatography, DE52 column chromatography, and Mono Q column chromatography	Plexaura homomalla

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arachidonic acid + O2	-	Plexaura homomalla	(8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid	-	?

Synonyms

Synonyms	Comment	Organism
8R-lipoxygenase	-	Plexaura homomalla
8R-LOX	-	Plexaura homomalla

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Release 2023.1 (January 2023)