Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
A112D | the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates | Corynebacterium glutamicum |
A112G | the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates | Corynebacterium glutamicum |
A112I | the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates | Corynebacterium glutamicum |
A112S | the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
salicylate | - |
Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
2,5-Dihydroxybenzoate | at pH 8.0 and 25°C | Corynebacterium glutamicum | |
0.99 | - |
5-Fluorosalicylate | at pH 8.0 and 25°C | Corynebacterium glutamicum | |
2.7 | - |
5-Aminosalicylate | at pH 8.0 and 25°C | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | Corynebacterium glutamicum | the enzyme is highly specific for 2,5-dihydroxybenzoate | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | Corynebacterium glutamicum ATCC 13032 | the enzyme is highly specific for 2,5-dihydroxybenzoate | maleylpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NLB9 | - |
- |
Corynebacterium glutamicum ATCC 13032 | Q8NLB9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | the enzyme is highly specific for 2,5-dihydroxybenzoate | Corynebacterium glutamicum | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | the enzyme is highly specific for 2,5-dihydroxybenzoate | Corynebacterium glutamicum ATCC 13032 | maleylpyruvate | - |
? | |
5-aminosalicylate + O2 | - |
Corynebacterium glutamicum | ? | - |
? | |
5-aminosalicylate + O2 | - |
Corynebacterium glutamicum ATCC 13032 | ? | - |
? | |
5-fluorosalicylate + O2 | - |
Corynebacterium glutamicum | ? | - |
? | |
5-fluorosalicylate + O2 | - |
Corynebacterium glutamicum ATCC 13032 | ? | - |
? | |
additional information | no activity with salicylate, 3-methylsalicylate, 4-methylsalicylate, 5-methylsalicylate, 3-bromosalicylate, 4-bromosalicylate, 5-iodosalicylate, and 1-hydroxy-2-naphthoate | Corynebacterium glutamicum | ? | - |
? | |
additional information | no activity with salicylate, 3-methylsalicylate, 4-methylsalicylate, 5-methylsalicylate, 3-bromosalicylate, 4-bromosalicylate, 5-iodosalicylate, and 1-hydroxy-2-naphthoate | Corynebacterium glutamicum ATCC 13032 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDO | - |
Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
5-Fluorosalicylate | at pH 8.0 and 25°C | Corynebacterium glutamicum | |
1.1 | - |
5-Aminosalicylate | at pH 8.0 and 25°C | Corynebacterium glutamicum | |
2310 | - |
2,5-Dihydroxybenzoate | at pH 8.0 and 25°C | Corynebacterium glutamicum |