Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.4 extracted from

  • Hirano, S.; Morikawa, M.; Takano, K.; Imanaka, T.; Kanaya, S.
    Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W (2007), Biosci. Biotechnol. Biochem., 71, 192-199.
    View publication on PubMed
Search for more literature for 1.13.11.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Xanthobacter polyaromaticivorans

Inhibitors

Inhibitors Comment Organism Structure
1-hydroxy 2 naphthoate 0.33 mM, 19% of wild type enzyme activity remaining Xanthobacter polyaromaticivorans
Fe 2 mM, complete inactivation Xanthobacter polyaromaticivorans
salicylate 0.33 mM, 38% of wild type enzyme activity remaining Xanthobacter polyaromaticivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0186
-
 2,5-Dihydroxybenzoate
-
 Xanthobacter polyaromaticivorans
0.0209
-
 1-hydroxy 2 naphthoate
-
 Xanthobacter polyaromaticivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron ferrous ion specifically functions in the formation of the active tetramer structure, the enzyme contains 0.98 mol iron per mol subunit Xanthobacter polyaromaticivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
 4 * 36500, gel filtration Xanthobacter polyaromaticivorans
39300
-
 calculated from amino acid sequence Xanthobacter polyaromaticivorans
40000
-
 SDS-PAGE Xanthobacter polyaromaticivorans
146000
-
 gel filtration Xanthobacter polyaromaticivorans

Organism

Organism UniProt Comment Textmining
Xanthobacter polyaromaticivorans Q75W71
-
-
Xanthobacter polyaromaticivorans 127W Q75W71
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrapQ column chromatography Xanthobacter polyaromaticivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the specific activity of the purified recombinant enzyme is 102 units/mg at pH 7.5 Xanthobacter polyaromaticivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-dihydroxy-2-naphthoate + O2
-
 Xanthobacter polyaromaticivorans ?
-
 ?
1,4-dihydroxy-2-naphthoate + O2
-
 Xanthobacter polyaromaticivorans 127W ?
-
 ?
1-hydroxy-2-naphthoate + O2 weak activity Xanthobacter polyaromaticivorans ?
-
 ?
1-hydroxy-2-naphthoate + O2 weak activity Xanthobacter polyaromaticivorans 127W ?
-
 ?
2,5-dihydroxybenzoate + O2
-
 Xanthobacter polyaromaticivorans maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2
-
 Xanthobacter polyaromaticivorans 127W maleylpyruvate
-
 ?
5-aminosalicylate + O2 partly preferred substrate Xanthobacter polyaromaticivorans ?
-
 ?
5-aminosalicylate + O2 partly preferred substrate Xanthobacter polyaromaticivorans 127W ?
-
 ?
additional information no activity against catechol, benzoate, 5-methylsalicylate, 5-chlorosalicylate, and homogentisate Xanthobacter polyaromaticivorans ?
-
 ?
additional information no activity against catechol, benzoate, 5-methylsalicylate, 5-chlorosalicylate, and homogentisate Xanthobacter polyaromaticivorans 127W ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 36500, gel filtration Xanthobacter polyaromaticivorans

Synonyms

Synonyms Comment Organism
GDO
-
 Xanthobacter polyaromaticivorans
gentisate dioxygenase
-
 Xanthobacter polyaromaticivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme loses almost all its activity after incubation at 50°C for 15 min Xanthobacter polyaromaticivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.565
-
 1-hydroxy 2 naphthoate
-
 Xanthobacter polyaromaticivorans
5.5
-
 2,5-Dihydroxybenzoate
-
 Xanthobacter polyaromaticivorans
6.08
-
 1-hydroxy 2 naphthoate
-
 Xanthobacter polyaromaticivorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 GDO loses its activity after being kept in buffer below pH 6.5 for 12 h in the absence of ferrous iron and cysteine Xanthobacter polyaromaticivorans
8
-
 GDO loses its activity after being kept in buffer above pH 8.0 for 12 h in the absence of ferrous iron and cysteine Xanthobacter polyaromaticivorans

pI Value

Organism Comment pI Value Maximum pI Value
Xanthobacter polyaromaticivorans calculated from amino acid sequence
-
 5.4