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Literature summary for 1.13.11.39 extracted from

  • Bian, L.; Shuai, J.J.; Xiong, F.; Peng, R.H.; Yao, Q.H.; Xiong, A.S.
    Expression, purification, and characterization of a 2,3-dihydroxybiphenyl-1,2-dioxygenase from Bacillus sp. JF8 in Escherichia coli (2012), Biochem. Biophys. Res. Commun., 419, 339-343.
    View publication on PubMed
Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment) Organism
gene BsbphCI, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, the transgenic Escherichia coli strain expressing the BsbphCI gene degrades biphenyl-2,3-diol more quickly than the wild-type strain Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Bacillus sp. (in: Bacteria)
Cr3+
-
 Bacillus sp. (in: Bacteria)
Fe2+
-
 Bacillus sp. (in: Bacteria)
H2O2 81% inhibition at 10 mM Bacillus sp. (in: Bacteria)
KI 43% inhibition at 600 mM Bacillus sp. (in: Bacteria)
Li+
-
 Bacillus sp. (in: Bacteria)
SDS 46% inhibition at 25 mM Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates Bacillus sp. (in: Bacteria)
Co2+ activates Bacillus sp. (in: Bacteria)
Cu2+ activates Bacillus sp. (in: Bacteria)
K+ activates Bacillus sp. (in: Bacteria)
Mg2+ activates Bacillus sp. (in: Bacteria)
Ni2+ activates Bacillus sp. (in: Bacteria)
Zn2+ activates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Bacillus sp. (in: Bacteria)
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
biphenyl-2,3-diol + O2 Bacillus sp. (in: Bacteria) JF8
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q8GR45 gene BsbphCI
-
Bacillus sp. (in: Bacteria) JF8 Q8GR45 gene BsbphCI
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biphenyl-2,3-diol + O2
-
 Bacillus sp. (in: Bacteria) 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
biphenyl-2,3-diol + O2
-
 Bacillus sp. (in: Bacteria) JF8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
2,3-DHBP dioxygenase
-
 Bacillus sp. (in: Bacteria)
2,3-dihydroxybiphenyl-1,2-dioxygenase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 activity range Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified recombinant enzyme, 60 min, 33% activity remaining Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 recombinant enzyme Bacillus sp. (in: Bacteria)

General Information

General Information Comment Organism
physiological function 2,3-dihydroxybiphenyl-1,2-dioxygenase plays an important role in the degradation of polychlorinated biphenyls Bacillus sp. (in: Bacteria)