Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.39 extracted from

  • Iwata, K.; Nojiri, H.; Shimizu, K.; Yoshida, T.; Habe, H.; Omori, T.
    Expression, purification, and characterization of 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase from carbazole-degrader Pseudomonas resinovorans strain CA10 (2003), Biosci. Biotechnol. Biochem., 67, 300-7.
    View publication on PubMed
Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment) Organism
CarBaBb gene from Pseudomonas resinovorans strain CA10 expressed by expression vector pUCA503 in Escherichia coli BL21(DE3) Pseudomonas resinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 2'-aminobiphenyl-2,3-diol pH 7.5, 25°C Pseudomonas resinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE Pseudomonas resinovorans
29000
-
 alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE Pseudomonas resinovorans
78000
-
 heterotetramer, gel-filtration chromatography Pseudomonas resinovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas resinovorans
-
-
-
Pseudomonas resinovorans CA10
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-aminobiphenyl-2,3-diol + O2
-
 Pseudomonas resinovorans (2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H+
-
 ?
2'-aminobiphenyl-2,3-diol + O2
-
 Pseudomonas resinovorans CA10 (2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H+
-
 ?

Subunits

Subunits Comment Organism
heterotetramer alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE Pseudomonas resinovorans

Synonyms

Synonyms Comment Organism
2'-aminobiphenyl-2,3-diol 1,2-dioxygenase
-
 Pseudomonas resinovorans
CarB
-
 Pseudomonas resinovorans
CarBaBb
-
 Pseudomonas resinovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pseudomonas resinovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min full activity Pseudomonas resinovorans
60
-
 30 min, enzyme retains 30% activity Pseudomonas resinovorans
70
-
 30 min, enzyme completely inactivated Pseudomonas resinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudomonas resinovorans

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Pseudomonas resinovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
250
-
 2'-aminobiphenyl-2,3-diol pH 7.5, 25°C Pseudomonas resinovorans