Cloned (Comment) | Organism |
---|---|
expressed in Pseudomonas aeruginosa | Pseudomonas oleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | complete inactivation | Pseudomonas oleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
biphenyl-2,3-diol | pH 9.0, 25°C | Pseudomonas oleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required, after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
8 * 31000, SDS-PAGE | Pseudomonas oleovorans |
250000 | - |
PAGE | Pseudomonas oleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | - |
native enzyme and expressed in Pseudomonas aeruginosa | - |
Renatured (Comment) | Organism |
---|---|
after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine | Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87.2 | - |
pH 9.0, 25°C | Pseudomonas oleovorans |
Storage Stability | Organism |
---|---|
stable after addition of 10% acetone or 10% ethanol | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | - |
Pseudomonas oleovorans | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
additional information | no substrate: biphenyl-3,4-diol, catechol, 3-methylcatechol, 4-methylcatechol | Pseudomonas oleovorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | N-terminal amino acid sequence | Pseudomonas oleovorans |
octamer | 8 * 31000, SDS-PAGE | Pseudomonas oleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas oleovorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas oleovorans | isoelectric focusing | - |
4.5 |