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Literature summary for 1.13.11.37 extracted from

  • Kwon, D.; Suma, Y.; Tiwari, M.; Choi, J.; Kang, L.; Lee, J.; Kim, H.
    Decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase from Arthrobacter chlorophenolicus A6 and its structure characterization (2014), Int. Biodeter. Biodegrad., 95, 67-75 .
No PubMed abstract available
Search for more literature for 1.13.11.37

Application

Application Comment Organism
degradation the highly purified Ar 1,2-HQD can be used as a key enzyme in the biodegradation of aromatic hydrocarbon contaminants Pseudarthrobacter chlorophenolicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudarthrobacter chlorophenolicus
gene cphA-I, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudarthrobacter chlorophenolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxyquinol + O2 Pseudarthrobacter chlorophenolicus
-
 maleylacetate
-
 ?
hydroxyquinol + O2 Pseudarthrobacter chlorophenolicus A6 / DSM 12829
-
 maleylacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter chlorophenolicus
-
-
-
Pseudarthrobacter chlorophenolicus Q3BEM2
-
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829
-
-
-
Pseudarthrobacter chlorophenolicus A6 / DSM 12829 Q3BEM2
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA column chromatography Pseudarthrobacter chlorophenolicus
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration by 51.7-65.2fold from Escherichia coli strain BL21(DE3) Pseudarthrobacter chlorophenolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5
-
 recombinant enzyme, crude enzyme extract, substrate 4-chlorocatechol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
15.3
-
 recombinant enzyme, crude enzyme extract, substrate hydroxyquinol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
284.6
-
 51.7fold purified enzyme, with 4-chlorocatechol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
284.6
-
 purified recombinant enzyme, substrate 4-chlorocatechol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
391
-
 62.5fold purified enzyme, with catechol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
391
-
 purified recombinant enzyme, substrate catechol , pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
479.5
-
 purified recombinant enzyme, substrate 3-methylcatechol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus
479.6
-
 65.2fold purified enzyme, with 3-methylcatechol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
795.6
-
 52fold purified enzyme, with hydroxyquinol as substrate, at pH 7.5 and 25°C Pseudarthrobacter chlorophenolicus
795.6
-
 purified recombinant enzyme, substrate hydroxyquinol, pH 7.5, 25°C Pseudarthrobacter chlorophenolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
 Pseudarthrobacter chlorophenolicus ?
-
 ?
3-methylcatechol + O2
-
 Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
 ?
4-chlorocatechol + O2
-
 Pseudarthrobacter chlorophenolicus ?
-
 ?
4-chlorocatechol + O2
-
 Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
 ?
catechol + O2
-
 Pseudarthrobacter chlorophenolicus ?
-
 ?
catechol + O2
-
 Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
 ?
catechol + O2
-
 Pseudarthrobacter chlorophenolicus cis,cis-muconate
-
 ?
catechol + O2
-
 Pseudarthrobacter chlorophenolicus A6 / DSM 12829 cis,cis-muconate
-
 ?
hydroxyquinol + O2
-
 Pseudarthrobacter chlorophenolicus maleylacetate
-
 ?
hydroxyquinol + O2
-
 Pseudarthrobacter chlorophenolicus A6 / DSM 12829 maleylacetate
-
 ?
additional information decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase. Substrate specificity of Ar 1,2-HQD indicates that the catalytic function of this enzyme is similar to that of the type-II catechol 1,2-dioxygenase (1,2-CTD) Pseudarthrobacter chlorophenolicus ?
-
 ?
additional information decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase. Substrate specificity of Ar 1,2-HQD indicates that the catalytic function of this enzyme is similar to that of the type-II catechol 1,2-dioxygenase (1,2-CTD) Pseudarthrobacter chlorophenolicus A6 / DSM 12829 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000-37000, SDS-PAGE Pseudarthrobacter chlorophenolicus
? x * 35000-37000, recombinant His-tagged enzyme, SDS-PAGE, x * 33000-33500, about, His-tagged enzyme, sequence calculation Pseudarthrobacter chlorophenolicus
More enzyme Ar 1,2-HQD is an intradiol dioxygenase and it is related closely to 1,2-CTD. Its structure consisted of 5 alpha-helices in the N-terminal domain and 13 beta-sheets in the C-terminal domain. Three-dimensional enzyme structure homology modeling using structure PDB ID 1TMXA as template, overview Pseudarthrobacter chlorophenolicus

Synonyms

Synonyms Comment Organism
1,2-HQD
-
 Pseudarthrobacter chlorophenolicus
Ar 1,2-HQD
-
 Pseudarthrobacter chlorophenolicus
cphA-I
-
 Pseudarthrobacter chlorophenolicus
HQD
-
 Pseudarthrobacter chlorophenolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudarthrobacter chlorophenolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudarthrobacter chlorophenolicus

General Information

General Information Comment Organism
additional information three-dimensional enzyme structure homology modeling using structure PDB ID 1TMXA as template, overview Pseudarthrobacter chlorophenolicus
physiological function decomposition of aromatic hydrocarbon intermediates by recombinant hydroxyquinol 1,2-dioxygenase. Substrate hydroxyquinol is an intermediate found in the central pathway of biodegradation of various aromatic compounds by yeast, fungi, and bacteria Pseudarthrobacter chlorophenolicus