Literature summary for 1.13.11.37 extracted from

Zaborina, O.; Seitz, H.J.; Sidorov, I.; Eberspaecher, J.; Alexeeva, E.; Golovleva, L.; Lingens, F.
Inhibition analysis of hydroxyquinol-cleaving dioxygenases from the chlorophenol-degrading Azotobacter sp. GP1 and Streptomyces rochei 303 (1999), J. Basic Microbiol., 39, 61-73.

No PubMed abstract available

Activating Compound

Activating Compound	Comment	Organism	Structure
Fe(NH4)2(SO4)2	no activation by Fe2+ ions	Azotobacter sp.

Inhibitors	Comment	Organism
2,4-Dichlorophenol	mixed inhibition	Azotobacter sp.
2,4-Dichlorophenol	competitive inhibition	Streptomyces rochei
2,6-dichlorohydroquinone	competitive nhibition	Azotobacter sp.
2,6-dichlorohydroquinone	competitive inhibition	Streptomyces rochei
3,4,6-trichlorocatechol	mixed inhibition	Azotobacter sp.
3,4,6-trichlorocatechol	mixed inhibition	Streptomyces rochei
3,5-dichlorocatechol	competitive inhibition	Azotobacter sp.
3,5-dichlorocatechol	mixed inhibition	Streptomyces rochei
3-chlorocatechol	competitive inhibition	Azotobacter sp.
3-chlorocatechol	mixd inhibition	Streptomyces rochei
3-methyl-5-chlorocatechol	competitive inhibition	Azotobacter sp.
3-methyl-5-chlorocatechol	mixed inhibition	Streptomyces rochei
4,5-dichlorocatechol	competitive inhibition	Azotobacter sp.
4,5-dichlorocatechol	parially mixed competitive inhibition	Streptomyces rochei
4-Chlorocatechol	competitive inhibition	Azotobacter sp.
4-Chlorocatechol	competitive inhibition	Streptomyces rochei
catechol	mixed inhibition	Azotobacter sp.
EDTA	slight inhibition	Azotobacter sp.
monochlorohydroquinone	competitive inhibition	Azotobacter sp.
monochlorohydroquinone	competitive inhibition	Streptomyces rochei
pyrogallol	mixed inhibition	Azotobacter sp.
tetrachlorocatechol	mixed inhibition	Azotobacter sp.
tetrachlorocatechol	mixed inhibition	Streptomyces rochei
tetrachlorohydroquinone	competitive inhibition	Azotobacter sp.
tetrachlorohydroquinone	competitive inhibition	Streptomyces rochei
Tiron	-	Azotobacter sp.

Metals/Ions	Comment	Organism	Structure
Iron	1 mol of iron per mol enzyme	Streptomyces rochei
Iron	1 mol of iron per mol enzyme	Azotobacter sp.

Organism	UniProt	Comment	Textmining
Azotobacter sp.	-	grown with 2,4,6-trichlorophenol	-
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806	-	grown with 2,4,6-trichlorophenol	-
Streptomyces rochei	-	strain 303 VKM AC-1284D, grown with 2,4,6-trichlorophenol	-
Streptomyces rochei DSM 40242	-	strain 303 VKM AC-1284D, grown with 2,4,6-trichlorophenol	-

Purification (Comment)	Organism
-	Azotobacter sp.

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Azotobacter sp.

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.00069	-	pyrogallol	-	Azotobacter sp.
0.00083	-	monochlorohydroquinone	-	Streptomyces rochei
0.00107	-	monochlorohydroquinone	-	Azotobacter sp.
0.00128	-	tetrachlorocatechol	-	Streptomyces rochei
0.00226	-	tetrachlorohydroquinone	-	Streptomyces rochei
0.00349	-	tetrachlorocatechol	-	Azotobacter sp.
0.00536	-	3,4,6-trichlorocatechol	-	Streptomyces rochei
0.00585	-	4,5-dichlorocatechol	-	Streptomyces rochei
0.00589	-	2,6-dichlorohydroquinone	-	Streptomyces rochei
0.00698	-	3-chlorocatechol	-	Azotobacter sp.
0.0073	-	4-Chlorocatechol	-	Azotobacter sp.
0.0088	-	3,5-dichlorocatechol	-	Azotobacter sp.
0.00956	-	catechol	-	Azotobacter sp.
0.0103	-	4,5-dichlorocatechol	-	Azotobacter sp.
0.01094	-	2,6-dichlorohydroquinone	-	Azotobacter sp.
0.0112	-	3,4,6-trichlorocatechol	-	Azotobacter sp.
0.01248	-	tetrachlorohydroquinone	-	Azotobacter sp.
0.01274	-	2,4-Dichlorophenol	-	Azotobacter sp.
0.0169	-	3,5-dichlorocatechol	-	Streptomyces rochei
0.01719	-	2,4-Dichlorophenol	-	Streptomyces rochei
0.02732	-	3-methyl-5-chlorocatechol	-	Azotobacter sp.
0.03077	-	3-chlorocatechol	-	Streptomyces rochei
0.0373	-	3-methyl-5-chlorocatechol	-	Streptomyces rochei
0.0526	-	4-Chlorocatechol	-	Streptomyces rochei