BRENDA - Enzyme Database
show all sequences of 1.13.11.37

Purification and characterization of hydroxyquinol 1,2-dioxygenase from Azotobacter sp. strain GP1

Latus, M.; Seitz, H.J.; Eberspaecher, J.; Lingens, F.; Appl. Environ. Microbiol. 61, 2453-2460 (1995) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Fe(NH4)2(SO4)2
0.00001-0.000001 mM
Azotobacter sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Tiron
-
Azotobacter sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
0.18 mol per mol enzyme
Azotobacter sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34250
-
2 * 34250, SDS-PAGE
Azotobacter sp.
58000
-
gel filtration
Azotobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
benzene-1,2,4-triol + O2
Azotobacter sp.
-
3-hydroxy-cis-cis-muconate
-
Azotobacter sp.
?
benzene-1,2,4-triol + O2
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
3-hydroxy-cis-cis-muconate
-
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
Organism
Organism
UniProt
Commentary
Textmining
Azotobacter sp.
-
grown with 2,4,6-trichlorophenol
-
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
grown with 2,4,6-trichlorophenol
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Azotobacter sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.06
-
reactivated with 0.01 mM Fe2+
Azotobacter sp.
Storage Stability
Storage Stability
Organism
-80°C, no loss of activity after several weeks, at room temperature no considerable decrease after 1 day
Azotobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6-chlorohydroxyquinol + O2
33.5% relative activity of that measured with hydroxyquinol
439427
Azotobacter sp.
2-chloromaleylacetate
-
439427
Azotobacter sp.
?
6-chlorohydroxyquinol + O2
33.5% relative activity of that measured with hydroxyquinol
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
2-chloromaleylacetate
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp.
maleylacetate
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
439427
Azotobacter sp.
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
maleylacetate
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp.
3-hydroxy-cis-cis-muconate
-
439427
Azotobacter sp.
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
3-hydroxy-cis-cis-muconate
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 34250, SDS-PAGE
Azotobacter sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
in succinate buffer
Azotobacter sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Fe(NH4)2(SO4)2
0.00001-0.000001 mM
Azotobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Tiron
-
Azotobacter sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
0.18 mol per mol enzyme
Azotobacter sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34250
-
2 * 34250, SDS-PAGE
Azotobacter sp.
58000
-
gel filtration
Azotobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
benzene-1,2,4-triol + O2
Azotobacter sp.
-
3-hydroxy-cis-cis-muconate
-
Azotobacter sp.
?
benzene-1,2,4-triol + O2
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
3-hydroxy-cis-cis-muconate
-
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Azotobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.06
-
reactivated with 0.01 mM Fe2+
Azotobacter sp.
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, no loss of activity after several weeks, at room temperature no considerable decrease after 1 day
Azotobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6-chlorohydroxyquinol + O2
33.5% relative activity of that measured with hydroxyquinol
439427
Azotobacter sp.
2-chloromaleylacetate
-
439427
Azotobacter sp.
?
6-chlorohydroxyquinol + O2
33.5% relative activity of that measured with hydroxyquinol
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
2-chloromaleylacetate
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp.
maleylacetate
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
439427
Azotobacter sp.
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
maleylacetate
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp.
3-hydroxy-cis-cis-muconate
-
439427
Azotobacter sp.
?
benzene-1,2,4-triol + O2
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
3-hydroxy-cis-cis-muconate
-
439427
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 34250, SDS-PAGE
Azotobacter sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
in succinate buffer
Azotobacter sp.
Other publictions for EC 1.13.11.37
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742664
Kwon
-
Decomposition of aromatic hyd ...
Pseudarthrobacter chlorophenolicus, Pseudarthrobacter chlorophenolicus A6 / DSM 12829
Int. Biodeter. Biodegrad.
95
67-75
2014
-
1
2
-
-
-
-
-
-
-
-
4
-
6
-
-
2
-
-
-
10
-
18
3
5
1
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
2
-
-
10
-
18
3
1
-
-
-
1
-
-
-
-
2
2
-
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp., Pseudomonas sp. 1-7
BMC Microbiol.
12
27
2012
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
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-
-
-
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2
-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
711336
Wei
para-Nitrophenol 4-monooxygena ...
Pseudomonas sp.
Biodegradation
21
915-921
2010
-
-
1
-
-
-
-
-
1
-
1
-
-
5
-
-
1
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
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1
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1
-
1
-
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1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702491
Unell
Degradation of mixtures of phe ...
Pseudarthrobacter chlorophenolicus, Pseudarthrobacter chlorophenolicus A6 / DSM 12829
Biodegradation
19
495-505
2008
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
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1
-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706905
Dong
Characterization of a p-nitrop ...
Pseudomonas sp., Pseudomonas sp. PDS-7
Wei Sheng Wu Xue Bao
48
1486-1492
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
2
-
6
-
1
1
-
-
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1
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-
-
2
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
659477
Ferraroni
Crystal structure of the hydro ...
Pimelobacter simplex, Pimelobacter simplex 3E
J. Biol. Chem.
280
21144-21154
2005
-
-
1
1
-
-
1
1
-
1
-
2
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
1
1
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
671428
Nordin
Novel 4-chlorophenol degradati ...
Pseudarthrobacter chlorophenolicus A6
Appl. Environ. Microbiol.
71
6538-6544
2005
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
4
-
2
-
5
-
-
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-
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-
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-
-
2
-
-
1
-
-
-
-
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-
-
2
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659045
Kitagawa
A novel p-nitrophenol degradat ...
Rhodococcus opacus, Rhodococcus opacus SAO101
J. Bacteriol.
186
4894-4902
2004
-
-
1
-
-
-
-
-
-
-
1
-
-
12
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
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-
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1
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-
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-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439429
Zaborina
-
Inhibition analysis of hydroxy ...
Azotobacter sp., Streptomyces rochei, Streptomyces rochei DSM 40242, Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
J. Basic Microbiol.
39
61-73
1999
1
-
-
-
-
-
26
-
-
2
-
-
-
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
24
-
-
1
-
-
-
-
-
-
-
26
24
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439430
Murakami
Cloning of a gene encoding hyd ...
Arthrobacter sp.
Biosci. Biotechnol. Biochem.
63
859-865
1999
-
-
1
-
-
-
2
-
-
-
2
1
-
4
-
-
1
-
-
-
1
-
4
1
-
-
-
2
-
1
-
-
-
-
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-
1
-
-
-
-
-
2
-
-
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-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
439431
Benvenuti
-
Crystallization and preliminar ...
Pimelobacter simplex, Pimelobacter simplex 3E
Acta Crystallogr. Sect. D
55
901-903
1999
-
-
-
1
-
-
-
-
-
1
1
-
-
3
-
-
-
-
-
-
-
-
-
1
-
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-
-
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1
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-
-
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1
1
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390702
Daubaras
Purification of hydroxyquinol ...
Burkholderia cepacia
Appl. Environ. Microbiol.
62
4276-4279
1996
-
-
1
-
-
-
-
1
-
-
2
2
-
6
-
-
1
-
-
-
-
1
4
1
-
1
1
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
-
-
1
4
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
439426
Zaborina
Purification and characterizat ...
Streptomyces rochei, Streptomyces rochei DSM 40242
J. Bacteriol.
177
229-234
1995
-
-
-
-
-
-
-
2
-
1
2
2
-
3
-
-
1
-
-
-
1
1
6
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
2
-
-
-
1
-
-
1
1
6
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
439427
Latus
Purification and characterizat ...
Azotobacter sp., Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
Appl. Environ. Microbiol.
61
2453-2460
1995
1
-
-
-
-
-
1
-
-
1
2
2
-
10
-
-
1
-
-
-
1
1
6
1
-
-
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-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
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1
2
2
-
-
-
1
-
-
1
1
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439422
Boominathan
-
Dissimilation of aromatic subs ...
Arcopilus cupreus, Bipolaris oryzae, Fusarium oxysporum
Zentralbl. Mikrobiol.
144
37-45
1989
-
-
-
-
-
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3
-
3
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3
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6
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3
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3
-
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6
-
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-
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-
-
-
-
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-
-
-
-
439423
Sze
Properties of salicylate hydro ...
Cutaneotrichosporon cutaneum
J. Bacteriol.
159
353-359
1984
-
-
-
-
-
-
-
1
-
1
3
1
-
4
-
-
-
-
-
-
1
1
2
1
-
-
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-
-
1
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1
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1
3
1
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1
1
2
1
-
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-
-
1
-
-
-
-
-
-
-
-
-
439421
Buswell
-
Vanillic acid metabolism by se ...
Daedalea quercina, Fomes pinicola, Lenzites trabea, Polyporus dichrous, Poria ambigua, Trametes cinnabarina
Arch. Microbiol.
131
366-374
1982
-
-
-
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-
-
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6
-
6
-
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6
-
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12
-
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6
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6
-
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12
-
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-
-
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-
-
-
-
390689
Sparnins
Catabolism of L-tyrosine in Tr ...
Cutaneotrichosporon cutaneum
J. Bacteriol.
138
425-430
1979
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439424
Buswell
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Aromatic ring cleavage by the ...
Sporotrichum pulverulentum
FEBS Lett.
104
258-260
1979
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