Purification and characterization of hydroxyquinol 1,2-dioxygenase from Azotobacter sp. strain GP1

Latus, M.; Seitz, H.J.; Eberspaecher, J.; Lingens, F.; Appl. Environ. Microbiol. 61, 2453-2460 (1995)

Data extracted from this reference:

Activating Compound

Commentary

Organism

Structure

Fe(NH4)2(SO4)2

0.00001-0.000001 mM

Azotobacter sp.

Inhibitors

Commentary

Organism

Structure

Tiron

-

Azotobacter sp.

Metals/Ions

Commentary

Organism

Structure

Iron

0.18 mol per mol enzyme

Azotobacter sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

benzene-1,2,4-triol + O2

Azotobacter sp.

-

3-hydroxy-cis-cis-muconate

-

Azotobacter sp.

?

benzene-1,2,4-triol + O2

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

-

3-hydroxy-cis-cis-muconate

-

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

6-chlorohydroxyquinol + O2

33.5% relative activity of that measured with hydroxyquinol

439427

Azotobacter sp.

2-chloromaleylacetate

-

439427

Azotobacter sp.

?

6-chlorohydroxyquinol + O2

33.5% relative activity of that measured with hydroxyquinol

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

2-chloromaleylacetate

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp.

maleylacetate

maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate

439427

Azotobacter sp.

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

maleylacetate

maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp.

3-hydroxy-cis-cis-muconate

-

439427

Azotobacter sp.

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

3-hydroxy-cis-cis-muconate

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

Activating Compound

Commentary

Organism

Structure

Fe(NH4)2(SO4)2

0.00001-0.000001 mM

Azotobacter sp.

Inhibitors

Commentary

Organism

Structure

Tiron

-

Azotobacter sp.

Metals/Ions

Commentary

Organism

Structure

Iron

0.18 mol per mol enzyme

Azotobacter sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

benzene-1,2,4-triol + O2

Azotobacter sp.

-

3-hydroxy-cis-cis-muconate

-

Azotobacter sp.

?

benzene-1,2,4-triol + O2

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

-

3-hydroxy-cis-cis-muconate

-

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

6-chlorohydroxyquinol + O2

33.5% relative activity of that measured with hydroxyquinol

439427

Azotobacter sp.

2-chloromaleylacetate

-

439427

Azotobacter sp.

?

6-chlorohydroxyquinol + O2

33.5% relative activity of that measured with hydroxyquinol

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

2-chloromaleylacetate

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp.

maleylacetate

maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate

439427

Azotobacter sp.

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

maleylacetate

maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp.

3-hydroxy-cis-cis-muconate

-

439427

Azotobacter sp.

?

benzene-1,2,4-triol + O2

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

3-hydroxy-cis-cis-muconate

-

439427

Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

742664

Kwon

-

Decomposition of aromatic hyd ...

Pseudarthrobacter chlorophenolicus, Pseudarthrobacter chlorophenolicus A6 / DSM 12829

Int. Biodeter. Biodegrad.

95

67-75

2014

-

1

2

-

-

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4

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6

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2

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10

-

18

3

5

1

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1

2

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10

-

18

3

1

-

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-

1

-

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-

-

2

2

-

-

-

719179

Zhang

Identification of the para-nit ...

Pseudomonas sp., Pseudomonas sp. 1-7

BMC Microbiol.

12

27

2012

-

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1

1

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711336

Wei

para-Nitrophenol 4-monooxygena ...

Pseudomonas sp.

Biodegradation

21

915-921

2010

-

-

1

-

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1

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1

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5

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702491

Unell

Degradation of mixtures of phe ...

Pseudarthrobacter chlorophenolicus, Pseudarthrobacter chlorophenolicus A6 / DSM 12829

Biodegradation

19

495-505

2008

-

1

-

-

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4

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2

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-

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-

706905

Dong

Characterization of a p-nitrop ...

Pseudomonas sp., Pseudomonas sp. PDS-7

Wei Sheng Wu Xue Bao

48

1486-1492

2008

-

-

1

-

-

-

-

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7

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2

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6

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1

1

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6

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1

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-

659477

Ferraroni

Crystal structure of the hydro ...

Pimelobacter simplex, Pimelobacter simplex 3E

J. Biol. Chem.

280

21144-21154

2005

-

-

1

1

-

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1

1

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1

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2

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6

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1

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1

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1

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-

671428

Nordin

Novel 4-chlorophenol degradati ...

Pseudarthrobacter chlorophenolicus A6

Appl. Environ. Microbiol.

71

6538-6544

2005

-

-

2

-

1

-

-

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2

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3

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-

659045

Kitagawa

A novel p-nitrophenol degradat ...

Rhodococcus opacus, Rhodococcus opacus SAO101

J. Bacteriol.

186

4894-4902

2004

-

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1

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1

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12

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439429

Zaborina

-

Inhibition analysis of hydroxy ...

Azotobacter sp., Streptomyces rochei, Streptomyces rochei DSM 40242, Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

J. Basic Microbiol.

39

61-73

1999

1

-

-

-

-

-

26

-

-

2

-

-

-

6

-

-

1

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1

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24

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26

24

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-

439430

Murakami

Cloning of a gene encoding hyd ...

Arthrobacter sp.

Biosci. Biotechnol. Biochem.

63

859-865

1999

-

-

1

-

-

-

2

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2

1

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4

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1

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2

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1

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-

-

-

-

-

439431

Benvenuti

-

Crystallization and preliminar ...

Pimelobacter simplex, Pimelobacter simplex 3E

Acta Crystallogr. Sect. D

55

901-903

1999

-

-

-

1

-

-

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-

1

1

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3

-

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1

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1

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1

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-

390702

Daubaras

Purification of hydroxyquinol ...

Burkholderia cepacia

Appl. Environ. Microbiol.

62

4276-4279

1996

-

-

1

-

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-

-

1

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2

2

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6

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4

1

1

1

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1

1

1

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-

-

-

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-

439426

Zaborina

Purification and characterizat ...

Streptomyces rochei, Streptomyces rochei DSM 40242

J. Bacteriol.

177

229-234

1995

-

-

-

-

-

-

-

2

-

1

2

2

-

3

-

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1

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1

1

6

1

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1

6

1

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2

1

1

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-

439427

Latus

Purification and characterizat ...

Azotobacter sp., Azotobacter sp. GP1 / DSM 6428 / ATCC 49806

Appl. Environ. Microbiol.

61

2453-2460

1995

1

-

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1

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1

2

2

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10

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439422

Boominathan

-

Dissimilation of aromatic subs ...

Arcopilus cupreus, Bipolaris oryzae, Fusarium oxysporum

Zentralbl. Mikrobiol.

144

37-45

1989

-

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3

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3

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439423

Sze

Properties of salicylate hydro ...

Cutaneotrichosporon cutaneum

J. Bacteriol.

159

353-359

1984

-

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439421

Buswell

-

Vanillic acid metabolism by se ...

Daedalea quercina, Fomes pinicola, Lenzites trabea, Polyporus dichrous, Poria ambigua, Trametes cinnabarina

Arch. Microbiol.

131

366-374

1982

-

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6

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12

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390689

Sparnins

Catabolism of L-tyrosine in Tr ...

Cutaneotrichosporon cutaneum

J. Bacteriol.

138

425-430

1979

-

-

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439424

Buswell

-

Aromatic ring cleavage by the ...

Sporotrichum pulverulentum

FEBS Lett.

104

258-260

1979

-

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