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Literature summary for 1.13.11.37 extracted from
- Purification of hydroxyquinol 1,2-dioxygenase and maleylacetate reductase: the lower pathway of 2,4,5-trichlorophenoxyacetic acid metabolism by Burkholderia cepacia AC1100 (1996), Appl. Environ. Microbiol., 62, 4276-4279.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Burkholderia cepacia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
Hydroxyquinol | - |
Burkholderia cepacia |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, SDS-PAGE | Burkholderia cepacia |
| 68000 | - |
gel filtration | Burkholderia cepacia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzene-1,2,4-triol + O2 | Burkholderia cepacia | - |
3-hydroxy-cis-cis-muconate | - |
? | |
| benzene-1,2,4-triol + O2 | Burkholderia cepacia AC1100 | - |
3-hydroxy-cis-cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
AC1100, grown with 2,4,6-trichlorophenol | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for 2 to 3 weeks in elution buffer, can not be stored at 4°C or frozen at -20°C | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzene-1,2,4-triol + O2 | - |
Burkholderia cepacia | maleylacetate | - |
? | |
| benzene-1,2,4-triol + O2 | - |
Burkholderia cepacia AC1100 | maleylacetate | - |
? | |
| benzene-1,2,4-triol + O2 | - |
Burkholderia cepacia | 3-hydroxy-cis-cis-muconate | - |
? | |
| benzene-1,2,4-triol + O2 | - |
Burkholderia cepacia AC1100 | 3-hydroxy-cis-cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, SDS-PAGE | Burkholderia cepacia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Burkholderia cepacia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 52 | - |
Burkholderia cepacia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.35 | - |
Hydroxyquinol | - |
Burkholderia cepacia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Burkholderia cepacia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.2 | - |
Burkholderia cepacia |
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Release 2023.1 (January 2023)
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