General Stability | Organism |
---|---|
high osmotic sensitivity | Phenylobacterium immobile |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
high ionic strength | - |
Phenylobacterium immobile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
3-Methylpyrocatechol | - |
Phenylobacterium immobile | |
0.16 | - |
4-Methylpyrocatechol | - |
Phenylobacterium immobile | |
0.2 | - |
5-Amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone | - |
Phenylobacterium immobile | |
0.66 | - |
pyrocatechol | - |
Phenylobacterium immobile |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Fe2+ | Phenylobacterium immobile | |
Fe2+ | required for activity | Phenylobacterium immobile | |
Zn2+ | can partially replace Fe2+ | Phenylobacterium immobile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
6 * 34000, SDS-PAGE | Phenylobacterium immobile |
200000 | - |
analytical ultracentrifugation | Phenylobacterium immobile |
220000 | - |
gel filtration | Phenylobacterium immobile |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 | Phenylobacterium immobile | enzyme of pyrazone metabolism | 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phenylobacterium immobile | - |
DSM 1986 | - |
Purification (Comment) | Organism |
---|---|
- |
Phenylobacterium immobile |
Storage Stability | Organism |
---|---|
-20°C, stable for months | Phenylobacterium immobile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylpyrocatechol + O2 | - |
Phenylobacterium immobile | 2-hydroxy-3-methylmuconic acid semialdehyde | - |
? | |
4-methylpyrocatechol + O2 | - |
Phenylobacterium immobile | 2-hydroxy-3-methylmuconic acid semialdehyde | - |
? | |
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 | - |
Phenylobacterium immobile | 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone | - |
? | |
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 | enzyme of pyrazone metabolism | Phenylobacterium immobile | 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone | - |
? | |
catechol + O2 | - |
Phenylobacterium immobile | 2-hydroxymuconic acid semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 34000, SDS-PAGE | Phenylobacterium immobile |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Phenylobacterium immobile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Phenylobacterium immobile |