Literature summary for 1.13.11.34 extracted from

Ball, A.K.; Beilstein, K.; Wittmann, S.; Sueruen, D.; Saul, M.J.; Schnuetgen, F.; Flamand, N.; Capelo, R.; Kahnt, A.S.; Frey, H.; Schaefer, L.; Marschalek, R.; Haefner, A.K.; Steinhilber, D.
Characterization and cellular localization of human 5-lipoxygenase and its protein isoforms 5-LODELTA13, 5-LODELTA4 and 5-LOp12 (2017), Biochim. Biophys. Acta, 1862, 561-571 .

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Cloned(Commentary)

Cloned (Comment)	Organism
detection of alternatively spliced 5-LO transcripts in B and T cell lines and in primary cells encoding naturally occuring mutant 5-LO catalytically inactive isoforms 5-LODELTA13, 5-LODELTA4 and 5-LOp12, that lack the exons 13, 4 or a part of exon 12, respectively, RT-PCR expression analysis of 5-LO-WT and isoform expression. Coexpression of the truncated natural isoforms with 5-LO-wild-type in transiently and stably transfected HEK-293T cells	Homo sapiens

Cloned (Comment)

Organism

detection of alternatively spliced 5-LO transcripts in B and T cell lines and in primary cells encoding naturally occuring mutant 5-LO catalytically inactive isoforms 5-LODELTA13, 5-LODELTA4 and 5-LOp12, that lack the exons 13, 4 or a part of exon 12, respectively, RT-PCR expression analysis of 5-LO-WT and isoform expression. Coexpression of the truncated natural isoforms with 5-LO-wild-type in transiently and stably transfected HEK-293T cells

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	the naturally occuring mutant 5-LO catalytically inactive isoforms 5-LODELTA13, 5-LODELTA4 and 5-LOp12, lacking the exons 13, 4 or a part of exon 12, respectively, are identified in B and T cell lines as well as in primary B and T cells and monocytes. Wild-type 5-LO is localized in the nucleus whereas all natural mutant isoforms are located in the cytosol, Despite colocalization with the S271A mutant, the isoforms do not affect leukotriene biosynthesis. Coexpression of the truncated natural isoforms inhibits or stimulates 5-LO-wild-type expression in transiently and stably transfected HEK-293T cells suggesting that the isoforms have other functions than canonical leukotriene biosynthesis	Homo sapiens
S271A	site-directed mutagenesis, the mutation changes the localization of the wild-type enzyme to the cytosol	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-
additional information	wild-type 5-LO is localized in the nucleus whereas all natural truncation mutant isoforms and point mutant S271A are located in the cytosol. Treatment of the cells with sorbitol or KN-93/SB203580 changes the localization of the wild-type enzyme to the cytosol	Homo sapiens	-	-
nucleus	wild-type enzyme	Homo sapiens	5634	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
arachidonate + O2	Homo sapiens	-	(5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P09917	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	wild-type 5-LO shows a slight phosphorylation at S271 and S523, while mutant isozymes 5-LOp12 and 5-LODELTA13 are highly phosphorylated at Ser271 and 5-LOp12 also at Ser523	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
B-lymphocyte	-	Homo sapiens	-
eosinophil	-	Homo sapiens	-
granulocyte	eosinophil	Homo sapiens	-
macrophage	alveolar macrophage	Homo sapiens	-
monocyte	-	Homo sapiens	-
additional information	expression of wild-type 5-LO and particularly of the 5-LODELTA13 and 5-LOp12 mutant isoforms is increased in monocytes from patients with rheumatoid arthritis and sepsis	Homo sapiens	-
T-lymphocyte	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arachidonate + O2	-	Homo sapiens	(5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
5-lipoxygenase	-	Homo sapiens
5-LO	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	coexpression of the truncated natural isoforms inhibits or stimulates 5-LO-wild-type expression in transiently and stably transfected HEK-293T cells suggesting that the isoforms have other functions than canonical leukotriene biosynthesis	additional information

General Information

General Information	Comment	Organism
malfunction	the naturally occuring mutant 5-LO catalytically inactive isoforms 5-LODELTA13, 5-LODELTA4 and 5-LOp12, lacking the exons 13, 4 or a part of exon 12, respectively, are identified in B and T cell lines as well as in primary B and T cells and monocytes. Wild-type 5-LO is localized in the nucleus whereas all natural mutant isoforms are located in the cytosol. Coexpression of the truncated natural isoforms inhibits or stimulates 5-LO wild-type expression in transiently and stably transfected HEK-293T cells suggesting that the isoforms have other functions than canonical leukotriene biosynthesis. Detection of alternatively spliced 5-LO transcripts in primary monocytes of patients with rheumatoid arthritis and sepsis	Homo sapiens