Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.34 extracted from

  • Schroeder, M.; Haefner, A.K.; Hofmann, B.; Radmark, O.; Tumulka, F.; Abele, R.; Doetsch, V.; Steinhilber, D.
    Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase (2014), Biochim. Biophys. Acta, 1842, 1538-1547 .
    View publication on PubMed
Search for more literature for 1.13.11.34

Application

Application Comment Organism
medicine the enzyme is a promising target for anti-inflammatory therapy Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged wild-type enzyme, enzyme mutant, and isolated enzyme PLAT domain in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the isolated PLAT W75G interacts with calcium and the Dicer fragment Homo sapiens
W75G site-directed mutagenesis, the mutation causes reduced aggregation and substantially increased thermal stability of the mutant. The mutant still shows Ca2+ interactions. Mutation W75G thermally stabilises the isolated PLAT domain Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
additional information membrane-binding residue tryptophan 75 Homo sapiens
-
-
nuclear membrane
-
 Homo sapiens 31965
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ allosteric modulator involved in membrane binding by the enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13800
-
 isolated enzyme PLAT domain, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonate + O2 Homo sapiens
-
 (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P09917
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type enzyme and enzyme mutant from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, isolated enzyme PLAT domain Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
 Homo sapiens (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
5-lipoxygenase
-
 Homo sapiens
5-LOX
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information the ability of the isolated PLAT domain to bind Dicer C-terminus whereas the interaction with coactosin-like protein requires the interplay of the catalytic and the PLAT domain Homo sapiens
physiological function 5-lipoxygenase (5-LOX) is the key player of pro-inflammatory leukotriene biosynthesis. Its regulatory or socalled PLAT (polycystin-1, lipoxygenase, alpha-toxin) domain binds allosteric modulators like calcium, membranes, coactosin-like protein and Dicer, thereby influencing 5-LOX activity at the nuclearmembrane by mediating translocation. The PLAT domain may also regulate cytosolic 5-LOX activity and possibly influence microRNA metabolism Homo sapiens