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Literature summary for 1.13.11.34 extracted from
- Archidonate 5-lipoxygenase of guinea pig peritoneal polymorphonuclear leukocytes (1983), J. Biol. Chem., 258, 5754-5758.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5,8,11,14,17-eicosapentaenoic acid | competitive inhibition of oxygenation of arachidonic acid | Cavia porcellus |  |
| adenosine | slight stimulation | Cavia porcellus | |
| ADP | stimulation | Cavia porcellus | |
| AMP | stimulation | Cavia porcellus | |
| ATP | stimulates | Cavia porcellus | |
| cAMP | stimulation | Cavia porcellus | |
| GTP | stimulation | Cavia porcellus | |
| UTP | stimulation | Cavia porcellus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,8,11,14,17-eicosapentaenoic acid | - |
Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
5,8,11,14,17-eicosapentaenoic acid | - |
Cavia porcellus | |
| 0.019 | - |
arachidonic acid | - |
Cavia porcellus | |
| 0.09 | - |
8,11,14-eicosatrienoic acid | - |
Cavia porcellus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Cavia porcellus | |
| Ca2+ | half-maximal activity at 0.02 mM | Cavia porcellus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukocyte | peritoneal polymorphonuclear | Cavia porcellus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 1 month, 50% loss of activity | Cavia porcellus |
| 0°C, 30 h, about 70% loss of activity, in presence of 2 mM ATP only 30% loss of activity | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,8,11,14,17-eicosapentaenoic acid + O2 | - |
Cavia porcellus | ? | - |
? | |
| 8,11,14-eicosatrienoic acid + O2 | - |
Cavia porcellus | ? | - |
? | |
| arachidonate + O2 | i.e. 5,8,11,14-eicosatetraenoic acid | Cavia porcellus | (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
5 min, stable up to | Cavia porcellus |
| 60 | - |
5 min, most of the activity is lost | Cavia porcellus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
5,8,11,14,17-eicosapentaenoic acid | - |
Cavia porcellus | |
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