Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.34 extracted from

  • Denis, D.; Falgueyret, J.P.; Riendeau, D.; Abramovitz, M.
    Characterization of the activity of purified recombinant human 5-lipoxygenase in the absence and presence of leukocyte factors (1991), J. Biol. Chem., 266, 5072-5079.
    View publication on PubMed
Search for more literature for 1.13.11.34

Activating Compound

Activating Compound Comment Organism Structure
protein factors from human leukocyte stimulates Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
high expression baculovirus system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
BW755C CAS: 66000-40-6; i.e. 3-amino-1-[3-(trifluoromethyl)phenyl]-2-pyrazoline, IC50: 0.013 mM Homo sapiens
L-656,224 i.e. 7-chloro-2-[(4-methoxyphenyl)-methyl]-3-methyl-5-propyl-4-benzofuranol, IC50: 0.0008 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ above 0.1 mM, required for maximal activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.006
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Homo sapiens (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid + 6-trans-leukotriene B4 + 12-epi-6-trans-leukotriene B4 ?
additional information the purified protein expresses both 5-oxygenase and leukotriene A4 synthase activities in the ratio of 6:1 Homo sapiens ?
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0008
-
 i.e. 7-chloro-2-[(4-methoxyphenyl)-methyl]-3-methyl-5-propyl-4-benzofuranol, IC50: 0.0008 mM Homo sapiens L-656,224
0.013
-
 i.e. 3-amino-1-[3-(trifluoromethyl)phenyl]-2-pyrazoline, IC50: 0.013 mM Homo sapiens BW755C