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Literature summary for 1.13.11.31 extracted from

  • Schaefer, M.; Fan, Y.; Gu, T.; Heydeck, D.; Stehling, S.; Ivanov, I.; Yao, Y.G.; Kuhn, H.
    The lipoxygenase pathway of Tupaia belangeri representing Scandentia. Genomic multiplicity and functional characterization of the ALOX15 orthologs in the tree shrew (2020), Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1865, 158550 .
    View publication on PubMed
Search for more literature for 1.13.11.31

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Tupaia chinensis

Protein Variants

Protein Variants Comment Organism
V418I mutant produces 21.5% of 12S- and 78.5% of 15S product Tupaia chinensis
V418I mutant produces 23.1% of 12S- and 76.9% of 15S product Tupaia chinensis
V418I/419M mutant produces 5.1% of 12S- and 94.9% of 15S product Tupaia chinensis
V418I/419M mutant produces 5.9% of 12S- and 94.1% of 15S product Tupaia chinensis
V418I/419M/353A mutant produces 89.6% of 12S- and 10.2% of 15S product Tupaia chinensis
V418I/419M/353A mutant produces 92.9% of 12S- and 7.1% of 15S product Tupaia chinensis
V418I/419M/353L mutant produces 16.9% of 12S- and 83.1% of 15S product Tupaia chinensis
V418I/419M/353L mutant produces 77.6% of 12S- and 22.4% of 15S product Tupaia chinensis
V419M mutant produces 5.46% of 12S- and 45.5% of 15S product Tupaia chinensis
V419M mutant produces 75.9% of 12S- and 24.1% of 15S product Tupaia chinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0185
-
 O2 pH 7.4, temperature not specified in the publication Tupaia chinensis
0.0874
-
 O2 pH 7.4, temperature not specified in the publication Tupaia chinensis
0.116
-
 arachidonate pH 7.4, temperature not specified in the publication Tupaia chinensis
0.232
-
 arachidonate pH 7.4, temperature not specified in the publication Tupaia chinensis

Organism

Organism UniProt Comment Textmining
Tupaia chinensis A0A8A0R1W6 isoform Alox15a
-
Tupaia chinensis A0A8A0R235 isoform Alox15c
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
 Tupaia chinensis (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoic acid
-
 ?
additional information enzyme additionally shows membrane oxygenase activity Tupaia chinensis ?
-
-

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE, x * 75155, calculated from sequence Tupaia chinensis
? x * 75000, SDS-PAGE, x * 75205, calculated from sequence Tupaia chinensis

Synonyms

Synonyms Comment Organism
Alox15
-
 Tupaia chinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
 Tupaia chinensis
25
-
-
 Tupaia chinensis