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Literature summary for 1.13.11.31 extracted from
- Human platelet 12-lipoxygenase, new findings about its activity, membrane binding and low-resolution structure (2008), J. Mol. Biol., 376, 193-209.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| low-resolution 16 A structure of enzyme in solution, dynamic light scattering and SAXS analysis of hp-12LOX shows that this enzyme as a whole is stable in the dimeric form, while mutants without PLAT domains associate into larger aggregates | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C7S | site-directed mutagenesis, the mutant shows highly reduced iron content compared to the wild-type enzyme, but exchange of the surface-exposed cysteine alters neither the catalytic activity nor the reaction specificity | Homo sapiens |
| additional information | truncation of the N-terminal PLAT domain impairs catalytic activity but does not alter reaction specificity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | dependence of the recombinant His-tagged isozyme hp-12LOX enzymatic reaction rate on the concentration of arachidonic acid and pH, kinetics, overview | Homo sapiens | |
| 0.0072 | - |
arachidonic acid | cytosolic hp-12LOX, pH 7.4, recombinant wild-type enzyme | Homo sapiens |  |
| 0.0087 | - |
arachidonic acid | membrane hp-12LOX, pH 7.4, recombinant wild-type enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | platelet 12-lipoxygenase | Homo sapiens | 5829 | - |
| membrane | membrane binding activities of wild-type platelet 12-lipoxygenase and mutant enzymes at pH 7.4, role of the PLAT domain membrane binding, overview | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | iron content of wild-type and mutant enzymes, overview | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62650 | - |
x * 62650, recombinant no-PLAT-mutant, SDS-PAGE | Homo sapiens |
| 76500 | - |
2 * 76500, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
| 158000 | - |
about, recombinant His-tagged wild-type and mutant enzymes, gel filtration | Homo sapiens |
| 675000 | - |
about, recombinant no-PLAT-mutant, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + O2 | Homo sapiens | - |
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | further metabolized to 12(S)-hydroxyeicosatetraenoic acid | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P18054 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | further metabolized to 12(S)-hydroxyeicosatetraenoic acid | ? | |
| arachidonate + O2 | two molecules of arachidonic acid bind to one molecule of the enzyme, role of the PLAT domain in activity and membrane binding, overview | Homo sapiens | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 76500, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
| oligomer | x * 62650, recombinant no-PLAT-mutant, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hp-12LOX | - |
Homo sapiens |
| human platelet 12-lipoxygenase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8.7 | - |
Homo sapiens |
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